应用PCR和斑点杂交试验检测35例儿童急性淋巴细胞白血病(ALL)IgH和TcRr基因重排,并对9例完全缓解期患儿进行了微小残留病(MRD)动态监测,结果表明:双基因标记检查较单一基因检测MRD更具优越性,可降低假阴性率,有利于临床预知疾病复发。 Thirty-five children with acute lymphoblastic leukemia (ALL) IgH and TcRr gene rearrangements were detected by PCR and dot-blot hybridization. Dynamic monitoring of minimal residual disease (MRD) was performed in 9 children with complete remission. The results showed that: The examination is more superior than the single gene detection MRD, which can reduce the false negative rate, which is conducive to the clinical prediction of disease recurrence.