论文部分内容阅读
目的:建立测定人参次苷H滴丸中人参皂苷Rh1和Rh2含量的UPLC法。方法:采用Waters Acquity UPLC HSS T3分析柱(100 mm×2.1 mm,1.8μm),乙腈-水二元梯度洗脱模式,流速为0.46 mL·min-1,柱温35℃,紫外检测器检测,检测波长为203 nm,外标法对人参次苷H滴丸的主要成分进行定量分析。结果:人参皂苷Rh1和Rh2的分离效果较好,人参皂苷Rh1的线性方程为y=2.500×106x+3.080×102,r=1,在1.982~198.2μg·mL-1范围线性良好;人参皂苷Rh2线性方程为y=2.400×106x+2.042×103,r=0.999 9,在6.501~650.1μg·mL-1范围线性良好。平均加样回收率分别为99.66%和99.38%,RSD分别为0.34%(n=9)和0.35%(n=9)。结论:本方法前处理简单、准确、重复性好,且分析时间短,可以用于人参次苷H滴丸质量控制。
Objective: To establish a UPLC method for the determination of ginsenoside Rh1 and Rh2 in ginseng Hidoxin H drip pills. Methods: Waters Acquity UPLC HSS T3 analytical column (100 mm × 2.1 mm, 1.8 μm) was used. The acetonitrile-water binary gradient elution was performed at a flow rate of 0.46 mL · min-1. The column temperature was 35 ℃ and detected by ultraviolet detector. The detection wavelength was 203 nm. The external standard method was used to quantitatively analyze the main components of ginsenoside H drop pills. Results: The separation of ginsenoside Rh1 and Rh2 was better. The linear equation of ginsenoside Rh1 was y = 2.500 × 106x + 3.080 × 102, r = 1. The linear range of ginsenoside Rh1 was 1.982 ~ 198.2μg · mL-1. The linear equation was y = 2.400 × 106x + 2.042 × 103, r = 0.999 9, and linear in the range of 6.501-650.1 μg · mL-1. The average recoveries were 99.66% and 99.38%, respectively, with RSDs of 0.34% (n = 9) and 0.35% (n = 9), respectively. Conclusion: The method is simple, accurate, reproducible and has short analysis time. It can be used for quality control of ginsenoside H drop pills.