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采用噻唑蓝 (MTT)测定、3H TdR参入、流式细胞术、荧光偏振技术和软琼脂培养 ,观察了柠檬酸镧 (LaCit)对人肺癌细胞 (PG)、人胃癌细胞 (BGC 82 3 )的增殖、DNA合成、细胞周期、细胞膜脂流动性、软琼脂生长的影响。LaCit的浓度为 0 0 5 ,0 1,0 5 ,1mmol·L- 1 时可抑制肺癌细胞PG的增殖 :浓度为 0 5 ,1mmol·L- 1 时可抑制肺癌细胞PG ,DNA的合成 ,其G1 期细胞比例增加 ,S期细胞比例减少 ,细胞膜脂流动性降低 ,在软琼脂中的生长能力降低。而同样浓度的LaCit对胃癌细胞BGC 82 3除可降低其细胞膜脂流动性外 ,未见其他影响。
Mitochonium citrate (LaCit) was applied to human lung cancer cells (PG) and human gastric cancer cells (BGC 82 3 ) using thiazolyl blue (MTT) assay, 3H TdR incorporation, flow cytometry, fluorescence polarization and soft agar culture. Proliferation, DNA synthesis, cell cycle, cell membrane lipid fluidity, soft agar growth effects. When the concentration of LaCit was 0 05, 0 1,0 5, and 1 mmol·L-1, the proliferation of PG in lung cancer cells was inhibited. At a concentration of 0 5, 1 mmol·L -1 could inhibit the synthesis of PG and DNA in lung cancer cells. The proportion of cells in the G1 phase increased, the proportion of cells in the S phase decreased, the lipid membrane fluidity of the cells decreased, and the ability to grow in soft agar decreased. However, the same concentration of LaCit had no effect on the cell membrane lipid fluidity of gastric cancer cells BGC 82 3 .