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1 引言 目前,临床上常见的新型胃药中均不同程度地含有一定量铋。长期服用,铋会在人体中蓄集,达一定量时可引起一系列副作用。因此,寻找一种较为简便适用的检测血液中铋含量的方法,对于药物的安全使用和疾病的诊断是很有帮助的。用于测定铋的方法有比色分析法、示波极谱法等。用碳糊电极示波极谱阳极溶出法测定铋尚未见报道。本文选用了可以避免汞污染的碳糊电极作为工作电扳,示波极谱阳极溶出测定铋含量,方法应用于服用含铋剂药物患者的血液测定,结果良好。 2 实验部分 2.1 主要仪器和试剂 JP-Ⅱ型示波极谱仪(成都仪器厂),自制碳糊电极,饱和甘汞电极为参比电极,铂丝为对电极。1×10~(-2)mol/LBiCl_3·2H_2O储备液(按常规方法配制,用时以1mol/L HCl溶液稀释所需浓度),石墨粉和石腊油为化学纯,其余所用试剂均为分析纯,水为二次蒸馏水。 2.2 实验方法 取一定量铋储备液于10ml小烧杯(电解池)中,加入1mol/L HCl 3ml,加水至10m1。仪器测量开关置“阳极溶出”处,原点电位为+0.3V,富集2min,立即溶出扫描,记录+0.14V(vs.SCE)处一阶导数峰高。 3 结果与讨论
1 Introduction At present, the new clinically common gastric drugs contain different levels of bismuth in varying degrees. Long-term use, bismuth accumulation in the body, up to a certain amount can cause a series of side effects. Therefore, looking for a simple and convenient method for detecting the content of bismuth in blood is very helpful for the safe use of drugs and the diagnosis of diseases. Methods for the determination of bismuth colorimetric analysis, oscillopolarography and so on. Determination of bismuth by oscillopolarographic anodic dissolution with carbon paste electrode has not been reported yet. In this paper, carbon paste electrode can be used to avoid mercury pollution as work pull, oscillopolarographic anodic dissolution of bismuth content determination, the method used in patients taking bismuth containing drugs blood test, the results are good. 2 Experimental 2.1 The main instruments and reagents JP-Ⅱ oscilloscope polarography (Chengdu Instrument Factory), homemade carbon paste electrode, saturated calomel electrode as the reference electrode, platinum wire as the counter electrode. The stock solution of 1 × 10 -2 mol / L LiCl 3 · 2H 2 O (prepared according to the conventional method and diluted with 1 mol / L HCl solution), graphite powder and paraffin oil were chemically pure, and the rest of reagents were analyzed Pure water is double distilled water. 2.2 Experimental Method Take a certain amount of bismuth stock solution in 10ml small beaker (electrolytic cell), add 1mol / L HCl 3ml, add water to 10ml. The instrument measuring switch was set to “anodic dissolution”, the origin potential was + 0.3V, enrichment 2min, immediately out of the scan, record + 0.14V (vs.SCE) first derivative peak height. 3 Results and discussion