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目的探讨血管内皮生长因子C(VEGF-C)在维吾尔族宫颈病变[宫颈上皮内瘤变(CIN)Ⅰ~Ⅲ及早期宫颈癌]组织中的表达及其与人乳头瘤病毒(HPV)载量相关性。方法采用实时荧光定量PCR(Real-time PCR)法测定140例维吾尔族妇女(慢性宫颈炎30例,CINⅠ、CINⅡ、CINⅢ各20例,早期宫颈癌50例)宫颈石蜡切片组织中VEGF-C的水平;术前用第2代杂交捕获(HC-Ⅱ)方法测定同一患者术前宫颈HPV载量;比较2个指标同宫颈病变的关联。结果 CINⅡ、CINⅢ及早期宫颈癌组织VEGF-C水平、宫颈病变程度及HPV载量均高于慢性宫颈炎组织(P<0.05);组织中VEGF-C水平与HPV载量呈正相关,差异有统计学意义(P<0.05)。结论新疆维吾尔族妇女宫颈组织中VEGF-C水平、HPV载量均与宫颈病变严重程度相关,且二者存在协同作用。
Objective To investigate the expression of vascular endothelial growth factor C (VEGF-C) in Uygur cervical lesions (cervical intraepithelial neoplasia (CIN) Ⅰ ~ Ⅲ and early cervical cancer] and its relationship with human papillomavirus (HPV) Correlation. Methods Real-time quantitative PCR (Real-time PCR) was used to detect the expression of VEGF-C in 140 cases of Uyghur women (30 cases of chronic cervicitis, 20 cases of CINⅠ, CINⅡ, CINⅢ and 50 cases of early stage cervical cancer) The preoperative cervical hybridization (HC-Ⅱ) method was used to determine the HPV load in the same patient before surgery. The correlation between the two indexes and cervical lesions was compared. Results The levels of VEGF-C, cervical lesion and HPV in CINⅡ, CINⅢ and early stage cervical cancer tissues were higher than those in chronic cervicitis tissues (P <0.05). There was a positive correlation between VEGF-C level and HPV load Significance (P <0.05). Conclusion The levels of VEGF-C and HPV load in cervical tissues of Uighur women in Xinjiang are related to the severity of cervical lesions, and synergistic effect exists between the two.