论文部分内容阅读
为测定分析中国第一例输入性中东呼吸综合征冠状病毒基因组序列.采用PCR分段扩增、测序、拼接的方法,组装病毒基因组序列,应用BLAST,MEGA6.0,SIMPLOT,RDP等软件分析病毒序列的相似性、变异、进化、重组事件.结果发现,所完成的病毒序列长度为29928 bp,与韩国报道的序列最为接近.病毒核苷酸序列与近期中东地区分离到的病毒株序列相似性高达99.53%~99.92%;在多聚蛋白ORF1ab中有3个新发突变,而S,E,M,N蛋白未出现变异;核苷酸序列无明显的片段重组现象.本研究表明,中国广东第一例输入性MERS患者的序列没有出现明显的变异,一些位点突变后功能变化仍需进一步研究.
To detect and analyze the first case of imported SARS-CoV genome in China, the virus genome sequences were assembled by PCR amplification, sequencing and splicing, and the viruses were analyzed by BLAST, MEGA6.0, SIMPLOT and RDP software Sequence similarity, mutation, evolution and recombination events.It was found that the length of the completed virus sequence was 29928 bp, which was closest to the sequence reported in S. Korea.The nucleotide sequence of the virus was similar to the sequence of the virus isolated recently in the Middle East Up to 99.53% ~ 99.92%. There were three new mutations in the polyprotein ORF1ab, but there was no variation in the S, E, M and N proteins.The nucleotide sequence showed no obvious fragment recombination.This study showed that Guangdong, China The first case of invasive MERS patients showed no significant sequence variation, functional changes after some sites still need further study.