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比较了异位性皮炎患者和正常对照组的嗜酸粒细胞对一系列化学趋化素的游走能力。26例符合 Hanifin 和 Rajka 诊断标准的异位性皮炎患者,至少对3种变应原过敏,总IgE 水平升高,对相关变应原的放射性变应性吸收试验(KAST)阳性。采血时,大多数患者有轻中度湿疹,局部有活动性皮损。所有患者外周血嗜酸粒细胞增多(>8%)。用两种不同的方法分离纯化异位性皮炎患者和正常对照组外周血的嗜酸粒细胞。用改良的 Boyden小室测定嗜酸粒细胞的游走性。化学趋化素
The ability of eosinophils to swim on a range of chemotactic chemokines was compared between patients with atopic dermatitis and controls. Twenty-six patients with atopic dermatitis who met the Hanifin and Rajka diagnostic criteria were allergic to at least three allergens and had elevated levels of total IgE, and were positive for the radio-allergic absorption test (KAST) of the relevant allergens. Blood collection, the majority of patients with mild to moderate eczema, local active lesions. Peripheral blood eosinophilia (> 8%) in all patients. Eosinophils were isolated and purified from peripheral blood of patients with atopic dermatitis and normal controls by two different methods. Eosinophil migration was determined using a modified Boyden chamber. Chemotoxins