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目的:研究麻黄甘草不同比例配伍前后水煎液中有效成分含量及其抗炎作用的变化,探讨两药的配伍规律。方法:采用GC-MS测定各水煎液中盐酸去甲基伪麻黄碱、盐酸去甲基麻黄碱、盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱的煎出量;采用HPLC测定各水煎液中甘草苷和甘草酸的煎出量。通过对醋酸所致小鼠腹腔毛细血管通透性增高的影响,来观察麻黄、甘草药对不同配伍配比水煎液的抗炎作用。结果:与单煎液相比,麻黄与甘草配伍后,随着配伍药材比例增大,水煎液中有效成分的含量均有不同程度的降低。与空白组比较,各给药组对醋酸所致小鼠腹腔毛细血管通透性增高均有不同程度地抑制作用,差异有统计学意义(P<0.05);与单味麻黄或甘草组比较,麻黄甘草(12:3)组和阳性对照组对醋酸所致小鼠腹腔毛细血管通透性增高均有抑制作用,差异有统计学意义(P<0.05),其余各配伍组均无统计学意义。结论:麻黄甘草配伍后,对彼此有效成分的溶出有一定的抑制作用。推断二药配伍后,甘草中有机酸和麻黄中生物碱之间可能发生了化学反应,生成难溶于水的生物碱有机酸复盐,导致配伍后水煎液中有效成分含量降低。麻黄甘草配伍组对醋酸所致小鼠腹腔毛细血管通透性增高有显著的抑制作用,随着甘草配伍比例的增加,抑制作用降低,推断是水煎液中有效成分含量降低所致。
OBJECTIVE: To study the changes of active ingredients and their anti-inflammatory effects in the decoction of Phyllostachys glycyrrhizae before and after compatibility with different proportions, and to explore the compatibility of the two drugs. Methods: The decocted amount of demethyl pseudoephedrine hydrochloride, demethylephedrine hydrochloride, ephedrine hydrochloride, pseudoephedrine hydrochloride and methyl ephedrine hydrochloride in each decoction was determined by GC-MS. Liquorice glycyrrhizin and licorice fried amount. Through the acetic acid-induced mouse peritoneal capillary permeability increased to observe Ephedra, licorice anti-inflammatory effect of different compatibility ratio decoction. Results: Compared with single decoction, after the compatibility of ephedra and licorice, with the increase of the ratio of medicinal herbs, the content of effective components in decoction decreased in varying degrees. Compared with the blank group, each administration group had inhibitory effect on the increase of peritoneal capillary permeability in mice induced by acetic acid, with statistical significance (P <0.05). Compared with the single ephedra or licorice group, Ephedra licorice (12: 3) group and positive control group had inhibitory effect on the increase of peritoneal capillary permeability in mice induced by acetic acid, the difference was statistically significant (P <0.05), the other compatibility groups had no statistical significance . Conclusion: The combination of Ma Huang Licorice, the dissolution of the active ingredient of each other have a certain inhibitory effect. It is inferred that after the compatibility of the two drugs, there may be a chemical reaction between the organic acids in the licorice root and the alkaloids in the ephedra, resulting in the organic acid double salt of the alkaloid hardly soluble in water, resulting in the decrease of the effective components in the decoction after compatibility. Ephedra licorice compatibility group on acetic acid-induced mice peritoneal capillary permeability increased significantly inhibited, with the licorice compatibility increased, the inhibitory effect decreased, inferred that the decoction of the active ingredient content decreased.