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目的探讨缺氧预处理(HPC)对缺氧缺血性脑损伤(HIBD)新生大鼠神经干细胞增殖的影响,为HPC的保护机制提供依据。方法 48只7 d龄新生SD大鼠,随机分成4组:缺氧缺血组、HPC+缺氧缺血组、假手术组、正常对照组,每组12只。缺氧缺血组在当天先行左侧颈总动脉结扎术,然后放入缺氧装置吸入8%氧气和92%氮气的混合气体2.5 h,建立HIBD动物模型。HPC+缺氧缺血组在术前1 d先行HPC,即将大鼠放入密闭容器中通入8%氧气和92%氮气混合气体3 h,第二天再依次同法建立HIBD动物模型。假手术组仅分离左侧颈总动脉,不结扎,也不做HPC。正常对照组未做任何处理。在建立动物模型后48 h将全部动物同时处死。对各组新生大鼠脑皮质、室管膜下区、海马区的神经干细胞增殖的标记物—巢蛋白(nestin)的表达进行观察。结果 HPC+缺氧缺血组在皮质、室管膜下区、海马区nestin阳性细胞数量明显多于其他3组(P<0.01或P<0.05),缺氧缺血组nestin阳性细胞数量也多于对照组与假手术组(P均<0.05),对照组nestin阳性细胞数量与假手术组比较无统计学差异(P>0.05)。结论 HPC对HIBD具有保护作用,可增加HIBD新生大鼠神经干细胞的增殖,以增加脑损伤的康复能力。
Objective To investigate the effect of hypoxic preconditioning (HPC) on the proliferation of neural stem cells in neonatal rats with hypoxic-ischemic brain damage (HIBD), and provide evidence for the protective mechanism of HPC. Methods 48 SD rats of 7 days old were randomly divided into 4 groups: hypoxia-ischemia group, HPC + hypoxia-ischemia group, sham operation group and normal control group, 12 rats in each group. Hypoxic ischemic group on the day before the first left common carotid artery ligation, and then into the hypoxia device inhaled 8% oxygen and 92% nitrogen gas mixture 2.5 h, the establishment of HIBD animal model. HPC + Hypoxia and Ischemia group were given HPC 1 day prior to surgery. The rats were placed in airtight container and infused with 8% oxygen and 92% nitrogen for 3 h. The next day, HIBD animal models were established in the same way. Sham-operated group only isolated left common carotid artery, not ligation, nor do HPC. The normal control group did not do any treatment. All animals were sacrificed 48 hours after establishment of the animal model. The expression of nestin, a marker of proliferation of neural stem cells in cerebral cortex, subependymal zone and hippocampus of neonatal rats in each group, was observed. Results The number of nestin positive cells in cortex, subependymal zone and hippocampus in HPC + hypoxia-ischemia group was significantly more than that in other three groups (P <0.01 or P <0.05), and the number of nestin positive cells in hypoxic-ischemic group was more than There was no significant difference in the number of nestin positive cells in the control group and the sham operation group (P> 0.05). Conclusion HPC has a protective effect on HIBD, which can increase the proliferation of neural stem cells in neonatal rats with HIBD and increase the rehabilitation ability of brain injury.