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This paper deals with the temperature correlation of gray scale of Bmode ultrasound image from heated tissue. In this study, many in-vitro fresh pig livers are heated in a temperature range from 28℃ to 45℃, from which a series of B-mode ultrasonic images of livers were obtained. The gray-value is evaluated from the ultrasound images respectively. A correlation of the mean gray value of the selected regions (12×12 pixels) in B-mode ultrasonic images of liver and its temperature was pointed out. And the experiment results agreed the evaluation well. And it is possible to monitor the tissue temperature changing in hyperthermia using this correlation.