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采用核团微量注射、推挽灌流结合行为测痛，神经肽的放免分析等实验方法分别从突触后受体和突触前递质释放的角度探讨了网状旁巨细胞核（ＲＰＧＬ）中的内阿片肽与针刺镇痛的关系。实验结果显示：大鼠ＲＰＧＬ微量注射纳洛酮（５μｇ／０．５μｌ／２ｍｉｎ）可部分翻转针刺镇痛效应，与电针加生理盐水组比较，注药后５ｍｉｎ、１０ｍｉｎ差异均有显著性意义（Ｐ＜０．０５）。ＲＰＧＬ内纳洛酮翻转针效的作用具有一定的剂量—效应关系。用放免测定的方法观察ＲＰＧＬ灌流液中内阿片肽含量在针刺前后的改变，显示电针组动物电针２０ｍｉｎ后，动物ＲＰＧＬ灌流液中亮氨酸脑啡肽（ＬＥＫ）的释放显著高于对照组（Ｐ＜０．０５）。其中高针效组动物电针后ＲＰＧＬ灌流液中β—内啡肽（β—ＥＰ）的释放高于对照组（Ｐ＜０．０５），而低针效组动物电针后β—ＥＰ的释放无明显增多。动物电针后的痛阈升高与ＲＰＧＬ中ＬＥＫ及β—ＥＰ的释放增多呈正相关（ｒ＝０．５２９，Ｐ＜０．０５；ｒ＝０．４８３，Ｐ＜０．０５）；电针２０ｍｉｎ后动物ＲＰＧＬ灌流液中强啡肽Ａ~（１～１３）（ＤｙｎＡ~（１～１３））的释放虽有所增加，但与对照组相比，无显著性意义（Ｐ＞０．０５）。上述结果分别从突触后受体?
The effects of microinjection of nuclei, push-pull perfusion combined with behavioral pain measurement, and radioimmunoassay of neuropeptides were used to investigate the effects of post-synaptic receptors and presynaptic neurotransmitters Relationship between endogenous opioid peptide and acupuncture analgesia. The experimental results showed that the microinjection of naloxone (5μg / 0.5μl / 2min) into the RPG of rats could partially reverse the analgesic effects of acupuncture. Compared with EA and saline groups, the difference was significant at 5min and 10min Significance (P <0.05). RPGL Naloxone flutter needle effect has a certain dose-effect relationship. Radioimmunoassay was used to observe the changes of the content of endogenous opioid peptide in RPGL perfusate before and after acupuncture. The results showed that the release of leucine enkephalin (LEK) in RPGL perfusate was significantly higher Control group (P <0.05). The release of β-EP in high-acupuncture group was higher than that in control group (P <0.05), while the low-acupuncture group had lower β-EP No significant increase in release. The increase of pain threshold after electroacupuncture was positively correlated with the increase of LEK and β-EP in RPGL (r = 0.529, P <0.05; r = 0.483, P <0.05) After 20 min, the release of DynA ~ (1 ~ 13) in RPGL perfusion fluid increased, but there was no significant difference compared with the control group (P> 0.05 ). The above results from the postsynaptic receptor?