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目的探讨大鼠心肌梗死重构过程中弹性纤维的变化及其机制和意义。方法SD大鼠冠状动脉前降支结扎形成心肌梗死模型,于术后2、4、8、12、21周时,用Masson染色、Uana地衣红染色技术和免疫组织化学方法,分别观察梗死区弹性纤维和白细胞介素1β(IL-1β)、肿瘤坏死因子(TNF-α)的变化,计算机图像分析系统作半定量分析。结果心肌梗死后2周梗死区即出现弹性纤维,至8~12周最高,21周弹性纤维明显减少或缺如,以胶原纤维为主。IL-1β、TNF-α表达在梗死后2周时可见,并随时间而增强。结论弹性纤维参与心肌梗死后的愈合过程,干预弹性纤维的形成可能成为抗心室重构的手段。
Objective To investigate the changes of elastic fibers during myocardial remodeling in rats and its mechanism and significance. Methods Myocardial infarction model was established by ligation of the anterior descending coronary artery of SD rats. Masson staining, Uva lichen red staining and immunohistochemical staining were performed at 2, 4, 8, 12 and 21 weeks after operation to observe the changes of infarction area elasticity Fiber and interleukin 1β (IL-1β), tumor necrosis factor (TNF-α) changes, computerized image analysis system for semi-quantitative analysis. Results Elastic fibers appeared in the infarct zone 2 weeks after myocardial infarction, and reached the peak at 8 to 12 weeks. At 21 weeks, the elastic fibers were significantly reduced or absent, mainly collagen fibers. IL-1β, TNF-α expression was visible at 2 weeks after infarction and increased with time. Conclusion Elastic fibers are involved in the healing process after myocardial infarction. Intervention of the formation of elastic fibers may be a means of anti-ventricular remodeling.