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本研究发现位于大肠杆菌不耐热肠毒素B亚单位(heat-labile enterotxin B subunit,LTB)的8肽(octapetptide,PT8A)具有一定的佐剂活性。我们用PT8A联合人手足口病病毒的VP1(EVP1)鼻腔免疫Balb/c小鼠,通过间接ELISA法检测小鼠血清中的特异性EVP1抗体滴度,检测结果显示PT8A+EVP1组相比PBS组和EVP1组有明显佐剂活性,初步验证了PT8A的佐剂活性。对PT8A和LTB蛋白的佐剂活性进行比较研究,结果显示,PT8A的佐剂活性明显弱于LTB,有待进一步提高。通过对PT8A免疫原性进行检测分析发现,PT8A的免疫原性明显减弱,初步确定PT8A在保留佐剂活性的同时自身免疫原性明显减弱,具有进一步开发为粘膜免疫佐剂候选分子的潜力。
This study found that octapetptide (PT8A) located in heat-labile enterotoxin B subunit (LTB) has certain adjuvant activity. We immunized Balb / c mice with VP8 (EVP1) of PT8A combined with human foot-and-mouth disease virus and detected the titer of specific EVP1 antibody in the serum of mice by indirect ELISA. The results showed that PT8A + EVP1 group And EVP1 group had obvious adjuvant activity, initially validated the adjuvant activity of PT8A. The adjuvant activities of PT8A and LTB proteins were compared. The results showed that the adjuvant activity of PT8A was significantly weaker than that of LTB, which needs to be further improved. Through the analysis of the immunogenicity of PT8A, the immunogenicity of PT8A was obviously weakened. It was preliminarily determined that the PT8A had an obvious decrease in the autoimmunogenicity while retaining the adjuvant activity, and had the potential to be further developed as a mucosal immune adjuvant candidate molecule.