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本研究观察了不同剂量γ－氨基丁酸（ＧＡＢＡ）对雄性大鼠下丘脑促性腺激素释放激素（ＧｎＲＨ）含量的作用。结果表明，脑室注射５、５０、２５０及５００ｍｍｏｌ／ＬＧＡＢＡ各为２０μｌ，４０分钟后下丘脑ＧｎＲＨ含量分别为３．５７±０．５８、３．７５±０．６６、４．６３±０．６３及５．０７±０．５９ｎｇ／１０ｍｇ湿重组织，均显著高于对照组下丘脑ＧｎＲＨ含量（１．７６±０．２１ｎｇ／１０ｍｇ湿重组织，Ｐ＜０．０５）。脑室注射２５０ｍｍｏｌ／ＬＧＡＢＡ２０μｌ后２０、４０、６０、１２０及２４０分钟下丘脑ＧｎＲＨ含量依次为２．２４±０．２９、４．６３±０．６３、５．１１±０．３８、４．２０±０．４０及３．９８±０．９１ｎｇ／１０ｍｇ湿重组织。ＧＡＢＡ受体拮抗剂荷包牡丹碱（ｂｉｃｕｃｕｌｉｎｅ，Ｂｉｃ）可阻断ＧＡＢＡ对下丘脑ＧｎＲＨ含量的促进作用。
This study investigated the effects of different doses of GABA on hypothalamic gonadotropin-releasing hormone (GnRH) content in male rats. The results showed that intraventricular injection of 5,50,250 and 500mmol / LGABA each 20μl, 40 minutes after hypothalamic GnRH content were 3.57 ± 0.58,3.75 ± 0.66,4.63 ± 0.63 And 5.07 ± 0.59ng / 10mg wet weight, respectively, were significantly higher than those in the control group (1.76 ± 0.21ng / 10mg wet weight, P <0.05). The contents of GnRH in the hypothalamus at 20, 40, 60, 120 and 240 minutes after intracerebroventricular injection of 20mmol / L LGABA were 2.24 ± 0.29,4.63 ± 0.63,5.11 ± 0.38 and 4.20 ± 0.40 and 3.98 ± 0.91 ng / 10 mg wet weight tissue. GABA receptor antagonist biculline (biciculine, Bic) can block GABA hypothalamic GnRH content.