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目的调查深圳地区2015年1月-12月致婴幼儿腹泻A组轮状病毒的分子流行病学特征及病毒基因亚型特点。方法收集深圳市2015年哨点医院的腹泻患儿粪便标本1 143例,进行轮状病毒核酸实时荧光定量PCR检测,对检测阳性的样本用巢式反转录PCR法进行G、P分型鉴定,应用SPSS 16.0软件进行统计分析。结果 2015年共收集哨点医院腹泻患儿粪便标本1 143例,其中轮状病毒阳性标本149例,感染率为13.03%,以0月龄~6月龄患儿所占比例最高;发病季节集中在春季、冬季(1月、12月);对其中141例RV阳性进行VP7血清型分型,G9型为优势株,占70%,其次为G1型、G2型、G3型,G3+G9混合型占10%;143例进行VP4基因分型,P[8]型为最流行的型别,占88%。结论轮状病毒是引起深圳地区婴幼儿腹泻的主要病原之一,主要流行株为G9P[8]型,与全国其他地区差异较大。这是深圳地区轮状病毒分型的首次报道。
Objective To investigate the molecular epidemiology and virus genotypes of group A rotavirus in infants with diarrhea in Shenzhen from January to December in 2015. Methods A total of 1 143 cases of diarrhea stool samples from sentinel hospitals in Shenzhen City in 2015 were collected for detection of rotavirus nucleic acid by real-time fluorescence quantitative PCR. The positive samples were identified by G, P typing by nested reverse transcription PCR , Using SPSS 16.0 software for statistical analysis. Results In 2015, a total of 1 143 cases of diarrhea in sentinel hospital were collected, of which 149 cases were positive for rotavirus. The infection rate was 13.03%, with the highest proportion of children aged 0 months to 6 months. The season of onset was concentrated In the spring and winter months (January and December), VP7 serotypes were positive in 141 cases of RV, the predominant strains of G9 were 70%, followed by G1, G2, G3 and G3 + G9 Type accounted for 10%; 143 cases of VP4 genotyping, P [8] type is the most popular type, accounting for 88%. Conclusions Rotavirus is one of the major pathogens causing infantile diarrhea in Shenzhen. The main epidemic strain is G9P [8], which is quite different from other parts of the country. This is the first report of rotavirus typing in Shenzhen.