目的通过检测Caspase3的活性反映Jurkat细胞凋亡。方法用放线菌素D诱导Jurkat细胞8h,裂解细胞,荧光分光光度计测定Caspase3的活性。同时,用Hoechst法进行检测,并进行平行的方法学比较。结果Ju-rkat细胞经不同浓度50μmol/L和100μmol/L的放线菌素D诱导后,Caspase3的活性显著增高,倍增值分别为10.091和10.818,相应的Caspase3活性单位为0.641和0.659;而未经诱导的Jurkat细胞仅为0.061。加入Cas-pase3抑制剂后,倍增值分别为0.101和0.119,Caspase3的活性单位为0.006和0.007。而用Hoechst法染色时,可见Jurkat细胞仅出现早期凋亡的形态学改变。结论荧光定量法是一种良好的定量检测Caspase3的方法,可用于检测早期的细胞凋亡。 Objective To detect the apoptosis of Jurkat cells by detecting the activity of Caspase  3. Methods Actinomycin D induced Jurkat cells 8h, cells were lysed, and the activity of Caspase-3 was measured by fluorescence spectrophotometer. At the same time, the Hoechst method was used for testing and parallel methodological comparison. Results Caspase3 activity was significantly increased in Ju-rkat cells induced by actinomycin D at concentrations of 50 μmol / L and 100 μmol / L, with multiplication values ​​of 10.091 and 10.818, respectively. The corresponding Caspase3 activity units were 0.641 and 0.659; whereas, Jurkat cells without induction were only 0.061. After adding Cas-pase  3 inhibitors, doubling values ​​were 0.101 and 0.119, Caspase  3 activity units 0.006 and 0.007. When Hoechst staining, Jurkat cells showed morphological changes of early apoptosis only. Conclusion Fluorescence quantitative method is a good quantitative detection of Caspase  3 method can be used to detect early apoptosis.