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透明质酸酶(Ⅰ)和注射用透明质酸酶(Ⅱ)的活力测定,通常用目视比浊法即“粘蛋白凝结”法,此法反应不专一,结果不准确。作者根据Ⅰ或Ⅱ所含的氨基葡糖脱水复合酶(Ⅲ)能促使透明质酸分解成低聚糖,并利用后者分子末端的氨基糖能与对-二甲氨基苯甲醛试剂反应显色的原理,设计新的光度测定法。作者并研究了透明质酸的酶解速度与其浓度、水解时间及其混合物与pH间关系;根据反应的最佳条件提出以下操作法: 取试管四只,二只作空白管,二只作测定管。空白管各加含0.15M氯化钠的0.1M醋酸盐缓冲液(pH=
The activity of hyaluronidase (I) and hyaluronidase (II) for injection is usually measured by the visual turbidimetric method called “mucin condensation”. The reaction is not specific and the result is inaccurate. According to Ⅰ or Ⅱ contained glucosamine dehydration enzyme (Ⅲ) can promote the decomposition of hyaluronic acid into oligosaccharides, and the use of the latter molecular amino sugar can react with p-dimethylaminobenzaldehyde reagent color The principle of designing a new photometric method. The authors also studied the relationship between the rate of enzymatic hydrolysis of hyaluronic acid and its concentration, hydrolysis time and its mixture with pH. According to the optimum reaction conditions, we proposed the following methods: take four test tubes, two blank tubes and two test tubes tube. Blank tubes were each supplemented with 0.1 M acetate buffer (pH = 0.15M NaCl)