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目的观察雌激素对绝经后雌性大鼠缺血性脑损伤的保护作用及机制。方法随机将12月龄雌性大鼠分为假手术组(A组)、对照组(B组)、雌激素处理组(C组)、雌激素+他莫西芬处理组(D组),采用线栓法制作大鼠大脑中动脉闭塞(MCAO)模型,观察缺血后24h脑梗死体积、神经功能缺损程度、血清NO及NOS浓度。结果雌激素处理组及雌激素+他莫西芬处理组均较对照组脑梗死体积小、神经功能缺损轻、血清NO及NOS浓度高;雌激素处理组较雌激素+他莫西芬处理组脑梗死体积小、神经功能缺损轻、血清NO及NOS浓度高。结论雌激素对绝经后雌性大鼠缺血性脑损伤具有明显的保护作用,时间窗为6h;雌激素提高血清NO及NOS浓度是神经保护作用的可能机制;雌激素受体拮抗剂他莫西芬可部分阻断雌激素的神经保护作用。
Objective To observe the protective effect and mechanism of estrogen on ischemic brain injury in postmenopausal female rats. Methods 12-month-old female rats were randomly divided into sham operation group (group A), control group (group B), estrogen treatment group (group C) and estrogen + tamoxifen treatment group (group D) The rat model of middle cerebral artery occlusion (MCAO) was established by thread occlusion. The volume of cerebral infarction, the degree of neurological deficit, serum NO and NOS concentration 24 h after ischemia were observed. Results Compared with the control group, the estrogen-treated group and the estrogen + tamoxifen-treated group had smaller cerebral infarction volume, less neurological impairment, and higher serum NO and NOS concentrations. Compared with the estrogen-treated group and the tamoxifen-treated group, Small cerebral infarction, neurological deficits, serum NO and NOS concentrations. Conclusion Estrogen has a protective effect on ischemic brain injury in postmenopausal female rats, with a time window of 6h. Estrogen can increase serum NO and NOS levels as a neuroprotective mechanism. Estrogen receptor antagonist, Fen can partially block the neuroprotective effect of estrogen.