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为动态比较土壤垦殖前后微生物群落代谢功能是否发生变化,利用Biolog-Eco技术对不同季节自然恢复(NR)和垦殖不同施肥处理[不施肥(NoF)、施化肥(CF)和化肥配施有机肥(CFM)]黑土微生物在4℃、15℃和28℃培养温度下的代谢功能多样性进行了研究。结果表明:4种处理在同一采样时期不同培养温度下,Biolog微孔板的单孔平均颜色变化率(Average Well Color Development,AWCD)值均表现为28℃>15℃>4℃,即微生物代谢活性随培养温度的升高而升高;同一采样时期样品在相同培养温度下黑土微生物对碳源的代谢能力总体呈现为NR>CFM>CF>NoF。微生物在积雪融化期和积雪覆盖期对碳源的利用能力高于植物生长季节。主成分分析(Principle Component Analysis,PCA)结果显示,不同采样时期NoF样品和其它3种处理相比微生物功能多样性差异较大;NR、CF和CFM在作物非生长季节(3月、4月和12月)的样品在PCA图中分散度小于在作物生长季节(6月)的样品。该研究结果综合表明,垦殖处理较自然恢复降低了土壤微生物群落对碳源的代谢能力,但并未改变微生物群落功能;不同季节土壤微生物功能多样性存在差异,NR、CF和CFM细菌群落在作物生长的夏季对碳源的利用能力差异最大。
In order to dynamically compare the metabolic functions of microbial communities before and after soil reclamation, Biolog-Eco technique was used to study the effects of different fertilization treatments (No Fertilization, No Fertilization, Fertilizer and Organic Fertilizer) (CFM)] Metabolic functional diversity of black soil microorganisms at 4 ° C, 15 ° C, and 28 ° C culture temperature was studied. The results showed that the average well Color Development (AWCD) values of Biolog microplates were 28 ℃> 15 ℃> 4 ℃ under the same culture temperature at different incubation periods, ie, microbial metabolism Activity increased with the increase of culture temperature. In the same sampling period, the metabolism ability of black soil microbes to carbon sources at the same culture temperature showed NR> CFM> CF> NoF. The ability of microorganisms to utilize carbon sources during snow melting and snow cover was higher than that during plant growth. The results of Principle Component Analysis (PCA) showed that there were significant differences in the functional diversity of NoF samples between different sampling periods and those of the other three treatments. In the non-growing season (March, April and April) December) samples were less dispersed in PCA plots than in the crop growing season (June). The results of this study show that the natural recovery of reclamation treatment reduced the microbial community carbon metabolism ability, but did not change the microbial community function. There were differences in functional diversity of soil microbes in different seasons, and the bacterial communities of NR, CF and CFM were under the control of crop Growing summer has the greatest difference in utilization of carbon sources.