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应用荧光法研究了中性溶液中吖啶橙与牛血清白蛋白分子间的结合反应 ,测定了结合常数KA 和结合位点数n ,讨论了荧光猝灭机理 ,依据F嵀ｒｓｔｅｒ非辐射能量转移机制确定了授体 -受体间的结合距离和能量转移效率 ,使用同步荧光技术考察了吖啶橙对牛血清白蛋白构象的影响 .图 3,参 11
The binding reaction between acridine orange and bovine serum albumin (BSA) in neutral solution was studied by fluorescence method. The binding constant KA and the number of binding sites n were determined. The fluorescence quenching mechanism was discussed. According to F 嵀 rster non-radiative energy transfer mechanism The donor-acceptor binding distance and energy transfer efficiency were determined, and the effect of acridine orange on the conformation of bovine serum albumin was investigated using the synchronous fluorescence technique.