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目的观察不同时长麦粒灸干预对去卵巢大鼠骨质疏松的作用及其初步机制。方法选取60只3月龄SD健康雌性大鼠,按随机数字表法分为假手术组和手术组,假手术组15只,手术组45只。除假手术组外采用双侧卵巢切除法,复制骨质疏松模型,术后连续10d以阴道图片检测动情周期筛选动物,复制成骨质疏松大鼠模型,从假手术组选取10只大鼠设为对照组,从手术组选取40只大鼠按随机数字表法分为模型组、雌激素组和麦粒灸一个半月组,麦粒灸三月组,每组10只。其中,对照组和模型组不作任何处理,雌激素组皮下注射戊酸雌二醇,麦粒灸组给予麦粒灸干预治疗。连续干预3个月后行骨密度(BMD)检测,然后从腹主动脉取血,检测血清雌二醇、碱性磷酸酶等指标。结果空白组、麦粒灸组和雌激素组大鼠腰椎(腰1~腰4)椎体和右侧股骨的BMD较模型组均有提高,但比较均无统计学意义(P>0.05);麦粒灸二组较麦粒灸一组比较,骨密度是提高的,其中两者的腰椎骨密度相比,P<0.05,两者有统计学差异;雌激素组的雌二醇和麦粒灸二组与模型组比较有显著性差异(P<0.01),麦粒灸二组的雌二醇和空白组比较有显著差异性(P<0.01),麦粒灸二组较麦粒灸一组比较也存在统计学差异(P<0.05)空白组碱性磷酸酶较模型组有显著差异性(P<0.01),麦粒灸二组较麦粒灸一组有明显降低,但差异无统计学意义。结论麦粒灸能明显提高去卵巢骨质疏松症模型大鼠腰椎骨密度和血清雌二醇的水平,下调血清碱性磷酸酶的水平,并且早期开始干预的效果明显优于晚期干预。
Objective To observe the effect of different duration of moxibustion on ovariectomized rats with osteoporosis and its primary mechanism. Methods Sixty three-month-old SD healthy female rats were randomly divided into sham operation group and operation group according to random number table method. There were 15 sham operation group and 45 operation group. In addition to the sham-operated group, bilateral ovariectomy was used to replicate the model of osteoporosis. The rats were screened for the estrous cycle by vaginal pictures 10 days after the operation to replicate osteoporosis rat models. Ten rats were selected from the sham operation group As control group, 40 rats were randomly divided into model group, estrogen group and moxibustion group for one and a half months, moxibustion group for March group, 10 rats in each group. Among them, the control group and model group without any treatment, estrogen subcutaneous injection of estradiol valerate, grain moxibustion group to give grain moxibustion intervention treatment. Bone mineral density (BMD) was measured 3 months after continuous intervention. Blood samples were taken from abdominal aorta and serum estradiol and alkaline phosphatase were measured. Results Compared with the model group, the BMD of the lumbar vertebra (lumbar 1 ~ lumbar 4) and the right femur of the blank group, the moxibustion group and the estrogen group were significantly increased (P> 0.05). Compared with the group of moxibustion, the bone mineral density of the two groups of grain moxibustion increased compared with that of the group of wheat moxibustion, in which the BMD of the lumbar vertebrae of the two groups was significantly higher than that of the group of moxibustion (P <0.05) There was significant difference between the two groups (P <0.01). There was significant difference between estradiol and blank group (P <0.01) (P <0.05). The alkaline phosphatase in the blank group was significantly different from that in the model group (P <0.01). The two groups of wheat granule moxibustion were significantly lower than those in the wheat granule moxibustion group, but the difference was not statistically significant . Conclusion Granule moxibustion can significantly improve lumbar BMD and serum estradiol level and serum alkaline phosphatase level in ovariectomized osteoporosis model rats, and the effect of early intervention is better than that of late intervention.