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为了构建HA2基因的新功能型重组乳酸菌,本试验通过PCR将来源于H9N2亚型禽流感病毒的血凝素基因HA2插入锚定表达载体p SIP409-pgsA’中,构建重组质粒pSIP409-pgsA’-HA2,并将其电转到植物乳杆菌NC8中,通过W estern-blot检测目的蛋白的表达情况。结果显示,成功构建了NC8-pSIP409-pgsA’-HA2,经Western-blot检测目的蛋白被成功表达,并且具有反应原性,目的条带大小与目的蛋白一致,为后期试验奠定了基础。
In order to construct new functional recombinant lactic acid bacteria of HA2 gene, HA2 gene of H9N2 avian influenza virus was inserted into the anchor expression vector p SIP409-pgsA ’by PCR to construct the recombinant plasmid pSIP409-pgsA’- HA2, and transferred to Lactobacillus plantarum NC8. The expression of the target protein was detected by Western-blot. The results showed that NC8-pSIP409-pgsA’-HA2 was successfully constructed. The target protein was successfully expressed by Western-blot and was reactive. The target band size was consistent with the target protein, which laid the foundation for the later experiment.