目的研究甘草Glycyrrhiza uralensis地上部分的化学成分。方法通过硅胶、凝胶等柱色谱及高效液相色谱技术进行分离纯化,根据理化性质和核磁数据对化合物进行结构鉴定。结果从甘草地上部分乙醇提取物中分离得到12个化合物,分别鉴定为(2S)-3′-(2-hydroxy-3-methylbut-3-enyl)-4′,5,7-trihydroxy-dihydroflavanone(1)、乔松素(2)、sigmoidin B(3)、licoflavanone(4)、6-异戊烯基柚皮素(5)、短叶松素(6)、高良姜素(7)、染料木素(8)、红车轴草素(9)、山柰酚-3-O-β-D-芸香糖苷(10)、芦丁(11)、α,α′-dihydro-3,5,3′-trihydroxy-4′-methoxy-5′-isopentenyl-stilbene(12)。结论化合物1为新化合物,命名为羟甘草黄烷酮;化合物3、6、7和9是首次从甘草属植物中分离得到。 Objective To study the chemical composition of the aerial part of Glycyrrhiza uralensis. Methods The compounds were isolated and purified by silica gel, gel column chromatography and high performance liquid chromatography (HPLC). The structures of compounds were identified by physico-chemical properties and NMR data. Results Twelve compounds were isolated from the ethanolic extracts of licorice root and identified as (2S) -3 ’- (2-hydroxy-3-methylbut-3-enyl) -4’, 5,7-trihydroxy-dihydroflavanone (6), galangin (7), genistein (8), genistein (2), sigmoidin B (3), licoflavanone (4), 6-isopentenyl naringenin ), Rutin (9), kaempferol-3-O-β-D-rutinoside (10), rutin (11), α, α’-dihydro-3,5,3’-trihydroxy- 4’-methoxy-5’-isopentenyl-stilbene (12). Conclusion Compound 1 is a new compound, named as Glycyrrhiza flavanone. Compounds 3, 6, 7 and 9 were isolated from Glycyrrhiza for the first time.