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选用分布于水稻(Oryza sativaL.)12条染色体上的36对SSR(simple sequence repeats)引物,分析了5个光温敏核不育系、7个细胞质雄性不育系及54份来自不同生态类型的恢复系或品种的遗传差异。在供试材料中共检测出300条多态性片段,平均每对SSR引物检测到8.33条。分析结果表明:(1)SSR标记明确地把供试的66份水稻材料中的65份区分开来,和已知系谱的亲缘关系多数吻合,与表型性状聚类结果也有一定的相似性。(2)以GD=0.73为标准,可准确地将籼稻、粳稻、野生稻三大类区分开;以GD=0.63为标准,又可区分出籼稻大类中带有粳稻或野生稻血缘的品种。表明SSR标记对籼稻、粳稻、野生稻品种的分类灵敏度高、结果可靠;并筛选出可用于鉴别水稻籼粳类型的10对特异性引物。(3)以GD=0.56为标准,将供试材料划分在8个生态类型中,为生产上杂优组合亲本的选配提供了一些有益的参考。同时,生产上常用的一些杂交稻亲本划分在不同的生态类型中,表明强优势组合亲本间的遗传差异一般较大。
Sixty-six pairs of simple sequence repeats (SSR) primers distributed on 12 chromosomes of rice (Oryza sativa L.) were used to analyze five thermo-sensitive genic male sterile lines, seven cytoplasmic male sterile lines and 54 genotypes from different ecotypes The genetic diversity of restorer lines or breeds. A total of 300 polymorphic fragments were detected in the tested materials, and an average of 8.33 pairs of SSR primers were detected. The results showed that: (1) The SSR markers clearly distinguished 65 of the 66 rice materials tested, most of them were related to the known pedigree, and similar to the clustering results of phenotypic traits. (2) According to GD = 0.73, the indica rice, japonica rice and wild rice can be distinguished accurately. GD = 0.63 is the standard to differentiate the varieties with indications of japonica or wild rice . The results showed that the SSR markers were highly sensitive to the classification of indica, japonica and wild rice cultivars, and the results were reliable. Ten pairs of specific primers that could be used to distinguish indica and japonica rice were screened out. (3) According to the standard of GD = 0.56, the tested materials were divided into eight ecotypes, which provided some useful references for the selection of hybrid parents in production. In the meantime, some hybrid rice parents commonly used in production are divided into different ecological types, indicating that the genetic differences among the parents with strong dominance are generally large.