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目的探讨宫颈癌组织中过氧化物还原酶4(Prdx4)蛋白表达变化及其对肿瘤增殖凋亡的影响。方法收集2013年6月-2016年9月长治医学院附属和济医院收治的宫颈癌患者90例作为观察组,另取同期在该院进行化验的宫颈炎患者58例作为对照组。检测入组患者宫颈标本组织中Prdx4蛋白表达量,根据观察组Prdx4蛋白表达量中位数进一步分为高Prdx4蛋白表达组、低Prdx4蛋白表达组各45例。采用RT-PCR法检测各组患者宫颈组织中的增殖、凋亡及自噬基因mRNA表达量。结果观察组患者宫颈Prdx4蛋白表达量显著高于对照组(P<0.05);高Prdx4蛋白表达组、低Prdx4蛋白表达组患者宫颈组织增殖基因内皮细胞特异性生长因子B2(ephrin B2)、缺氧诱导因子1α(HIF-1α)、肿瘤抗原2(Piwil2)mRNA表达量高于对照组,鸟苷酸结合蛋白1(GBP1)mRNA表达量低于对照组,Prdx4蛋白表达增加,增殖基因ephrin B2、HIT-1α、Piwil2 mRNA表达量上升,GBP1 mRNA表达量下降(P<0.05);高Prdx4蛋白表达组、低Prdx4蛋白表达组患者宫颈组织凋亡基因表皮脂肪酸结合蛋白-5(FABP-5)、肿瘤坏死因子α诱导的蛋白8(TNFAIP8)、存活蛋白(Survivin)、肽基脯氨酰顺反异构酶1(Pin1)mRNA表达量高于对照组,神经元衍生的孤核受体1(NOR1)mRNA表达量低于对照组,Prdx4蛋白表达量增加,凋亡基因FABP-5、TNFAIP8、Survivin、Pin1 mRNA表达量上升,NOR1 mRNA表达量下降(P<0.05);高Prdx4蛋白表达组、低Prdx4蛋白表达组患者宫颈组织自噬基因ARHI、Beclin1 mRNA表达量低于对照组,Cathepsin-D mRNA表达量高于对照组,Prdx4蛋白表达量增加,自噬基因ARHI、Beclin1 mRNA表达量降低,Cathepsin-D mRNA表达量上升(P<0.05)。结论 Prdx4蛋白表达增加可促使宫颈癌细胞增殖、抑制正常凋亡,是宫颈癌发生、发展的重要原因。
Objective To investigate the expression of Prdx4 in cervical cancer and its effect on the proliferation and apoptosis of tumor. Methods Ninety patients with cervical cancer who were admitted to Changzhi Medical College Affiliated and Ji’ning Hospital from June 2013 to September 2016 were selected as the observation group. Another 58 patients with cervicitis who were tested in the same hospital during the same period were selected as the control group. The expression of Prdx4 protein in cervical specimens was detected and divided into high Prdx4 protein expression group and low Prdx4 protein expression group according to the median of Prdx4 protein expression in observation group. The proliferation, apoptosis and expression of autophagy mRNA in cervical tissue of each group were detected by RT-PCR. Results The expression of Prdx4 protein in cervix of observation group was significantly higher than that of control group (P <0.05). In high Prdx4 protein expression group and low Prdx4 protein expression group, the expression of ephrin B2, The expression of HIF-1α and Piwil2 mRNA was higher than that of the control group. The expression of GBP1 mRNA was lower than that of the control group, while Prdx4 protein expression was increased. The expression of ephrin B2, The mRNA expression of HIT-1α and Piwil2 increased and the expression of GBP1 mRNA decreased (P <0.05). The expressions of apoptosis-related genes such as epidermal fatty acid binding protein-5 (FABP-5), apoptosis in cervical epithelial cells in high Prdx4 protein group and low Prdx4 protein group, The expression of tumor necrosis factor alpha induced protein 8 (TNFAIP8), Survivin, and Pin1 mRNA was higher than that of the control group. The number of neuron derived orphan nuclear receptor 1 ( (P <0.05). The expression of Prol4, Prol4, Prol4, Prol4, Prol4, Prol4, Prol4, Prol4 mRNA and Prdx4 mRNA were significantly higher in the Prdx4 protein group than in the control group Low Prdx4 protein expression in patients with cervical tissue autophagy gene ARHI, The expression of Beclin1 mRNA was lower than that of the control group, and the expression of Cathepsin-D mRNA was higher than that of the control group. The expression of Prdx4 protein increased, the expression of ARHI and Beclin1 mRNA decreased, and the expression of Cathepsin-D mRNA increased (P <0.05). Conclusion The increase of Prdx4 protein may promote the proliferation of cervical cancer cells and inhibit the normal apoptosis. It is an important reason for the occurrence and development of cervical cancer.