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目的 研究鲤鱼精巢DNA对细胞膜和线粒体的保护作用。方法 用H2 O2 致红细胞溶血 ,采用分光光度法检测溶血程度 ;用氧自由基损伤红细胞膜 ,采用Bialsche试剂直接比色法测定膜上的唾液酸含量 ;大鼠服用鲤鱼精巢DNA后 ,用荧光分光光度法检测其红细胞膜的流动性 ;用自由基发生体系损伤线粒体 ,采用分光光度法检测线粒体的膨胀度。结果 鲤鱼精巢DNA对H2 O2 致红细胞溶血有明显的对抗作用 ;鲤鱼精巢DNA可明显提高老化损伤红细胞膜上的唾液酸含量 ;大鼠服用鲤鱼精巢DNA后 ,其红细胞膜流动性明显增大 ;鲤鱼精巢DNA对Fe2 +-VitC体系所致的线粒体膨胀有明显的抑制作用。结论 鲤鱼精巢DNA可明显保护细胞膜和线粒体免受活性氧自由基的氧化损伤。
Objective To study the protective effect of salmon spermary DNA on cell membrane and mitochondria. Methods H2O2 induced red blood cell hemolysis, the degree of hemolysis was detected by spectrophotometry; red blood cell membrane was injured by oxygen free radicals, and the sialic acid content was determined by direct colorimetric method using Bialsche reagent; after the rats were administered salmon sperm DNA, fluorescence spectrophotometry was used. The erythrocyte membrane fluidity was detected by photometry; the mitochondria were damaged by the free radical generating system, and the swelling degree of mitochondria was measured by spectrophotometry. RESULTS: The testicular sperm DNA had obvious antagonistic effect on hemolysis of H2O2-induced erythrocytes. Testicular DNA of squid significantly increased the content of sialic acid on the membrane of aging-injured erythrocytes. After administration of salmon spermatozoa DNA, the erythrocyte membrane fluidity increased significantly. Testis DNA significantly inhibited mitochondrial swelling caused by the Fe2 +-VitC system. Conclusion The testis DNA of squid can obviously protect cell membrane and mitochondria against oxidative damage by reactive oxygen species.