建立了金银花中游离脂肪酸的微波快速萃取方法,采用柱前荧光衍生化法,测定了金银花中16种长链饱和与不饱和游离脂肪酸的含量。微波萃取功率800 W,溶剂V(氯仿):V(甲醇)=1:1,70℃萃取8 min,萃取率>98.8%。采用酸性乙腈/水流动相在反向C8色谱柱上进行梯度洗脱,HPLC荧光检测波长为505 nm,在线联用质谱辅助鉴定(APCI-MS),提高了检测的准确度。方法检出限在0.366~2.58μg/L之间,回收率88.3%~107.6%。 A rapid microwave extraction method for free fatty acids in honeysuckle was established. The contents of 16 kinds of long chain saturated and unsaturated fatty acids in honeysuckle were determined by the pre-column fluorescence derivatization. Microwave extraction power of 800 W, solvent V (chloroform): V (methanol) = 1: 1, 70 ℃ extraction 8 min, extraction rate> 98.8%. The gradient of acidic acetonitrile / water mobile phase was carried out on a reversed-phase C8 column with a fluorescence detection wavelength of 505 nm. The mass spectrometry-assisted determination (APCI-MS) was used online to improve the detection accuracy. The detection limit was 0.366 ~ 2.58μg / L, the recovery was 88.3% ~ 107.6%.