１９９５年７月对文县主要进行抽样免疫学监测，用尼龙管取血作ＥＬＩＳＡ共检测５２９人血清，阳性率３４％（１８／５２９），３１１岁儿童占９４１％（１７／１８），ＯＤ值平均１０９。６例有黑热病史的患者中仅１例为阳性。利什曼素皮试阳性率２３３％（１３０／５５９），男女之比为１０∶１２４，＜１２岁各年龄组阳性率均较高，＞１２岁全部阴性。皮试和ＥＬＩＳＡ并作的３９７人中，３例（３１％）均为阳性。经髂骨穿刺共检查家犬４７只，其中１只为阳性（２１％），将该犬骨髓液接种于金仓地鼠腹腔内，６个月后处死并检取小块肝、脾组织分别接种于２支３Ｎ培养基中，一周后培养结果阳性。对捕捉的白蛉进行解剖鉴定，结果是中华白蛉１００只（占９４３％）、歌乐山司蛉５只、鳞喙司蛉１只 In July 1995, Wenxian County was mainly sampled for immunological monitoring. 529 human serum samples were obtained by ELISA, and the positive rate was 34% (18/529). The percentage of children aged 311 years was 941% ( 17/18), and an OD of 1.09.6 was positive in only 1 of 6 patients with a history of kala-azar. Leishman skin test positive rate of 23  3% (130/559), male to female ratio of 1  1: 02424, <12 years old in all age groups were higher positive rates,> 12 years of all negative. Of the 397 patients who underwent skin test and ELISA, 3 (31%) were positive. A total of 47 dogs were examined by iliac puncture, of which 1 was positive (21%). The canine bone marrow was inoculated into the abdominal cavity of golden hamster. After 6 months, the dogs were sacrificed and seized a small piece of liver and spleen Tissues were inoculated into two 3N media, respectively, and the results were positive after one week. Anatomical identification of the catfish caught was performed. The result was 100 catfish (943%), 5 cats Gele Mountain, 1 snakehead