作者应用特异性DNA探针对口腔肿瘤17对染色体异常进行荧光标记原位杂交(FISH)技术检测研究。 研究对象源自山口大学附属医院口腔外科治疗的4例口腔肿瘤,其中疣状癌1例,粘膜表皮癌2例,多形性腺瘤1例,FISH技术操作基本按照Trask方法,其步骤:①PBS处理,②变性溶液处理,③杂交合剂调配,④冲洗液处理,⑤缓冲液清洗,⑥FITC荧光显色处理。 结果 本组4例显色程度不等,良性肿瘤显色较 The authors used specific DNA probes to detect 17 chromosomal abnormalities in oral tumors by fluorescence in situ hybridization (FISH) technique. The study included four cases of oral tumors treated by oral surgery in the Affiliated Hospital of Yamaguchi University. One case of verrucous carcinoma, two cases of mucosal epidermoid carcinoma and one case of pleomorphic adenoma were treated by FISH technique. The procedure was as follows: ①PBS treatment , ② denaturation solution treatment, ③ hybrid mixture preparation, ④ rinse solution processing, ⑤ buffer washing, ⑥ FITC fluorescence color processing. Results The group of 4 cases of varying degrees of color, benign tumor color more