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为培育耐碱的杂种砧木,以耐碱品种资阳香橙(Citrus junos Sieb.ex Tanaka)与不耐碱的枳[Poncirus trifoliata(L.)Raf.]杂交,将杂种F1群体经形态学与SSR鉴定杂种真实性,并通过叶片黄化程度分级、叶绿素与有效铁质量分数的测定以筛选耐碱杂种单株。结果表明,(1)与母本资阳香橙叶片形态上无明显差异的F1都是珠心苗。(2)真实杂种苗从第10片叶开始都有三出复叶。(3)筛选到2对SSR引物能鉴定出全部杂种苗。(4)在碱性土上,耐碱杂种苗叶绿素cha+b的质量分数显著低于耐碱亲本资阳香橙,而极显著高于不耐碱亲本枳;但叶绿素a的质量分数显著高于父母本。5、耐碱杂种叶片中有效铁质量分数显著高于枳。研究获得了2株耐碱能力较强的真实杂种苗。
In order to cultivate the alkali-tolerant hybrid rootstocks, hybrid F1 hybrids were crossed with Poncirus trifoliata (L.) Raf., An alkali tolerant variety, Citrus junos Sieb. The authenticity of the hybrids was identified and the hybrids of alkali-tolerant hybrids were screened by the determination of leaf yellowing degree, chlorophyll and available iron content. The results showed that: (1) There was no significant difference in the morphological characteristics of leaves of female Ziyang orange with F1. (2) The true hybrid seedlings have three compound leaves from the tenth leaf. (3) Two pairs of SSR primers were screened to identify all hybrid seedlings. (4) On alkali soil, the mass fraction of chlorophyll cha + b in alkali-tolerant hybrid seedlings was significantly lower than that in alkali-resistant parent Ziyang orange, but significantly higher than that in non-alkali resistant parent trifoliate orange. However, the mass fraction of chlorophyll a was significantly higher In the parents of this. 5, alkali-resistant hybrid leaves in the effective iron content was significantly higher than trifoliate orange. The study obtained two real hybrid seedlings with strong alkali resistance.