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酒精是一种亲脂性小分子物质,能迅速透过血脑屏障作用于脑,海马和小脑是脑内对酒精神经毒性最敏感的结构.本研究通过观测长期饮酒后小鼠海马内琥珀酸脱氢酶(succinate dehydroge-nase,SDH)含量的变化,以探讨酒精对脑能量代谢水平的影响.选用 15只 20-30g雄性昆明小鼠挺机均分为Ⅰ、Ⅱ、Ⅲ组.Ⅰ、Ⅱ、Ⅲ组分别以纯水、5%酒精、10%酒精为唯一饮料连续喂养60d后,取脑,振动切片机切片,组织化学 Peason法显示脑内SDH,图象分析仪测定海马CA_1、CA_3区SDH阳性反应物的平均光密度值(mean optical density,MOD).结果显示:CA1区SDH的MOD值 Ⅰ、Ⅱ、Ⅲ组分别为0.5329±0.0887、04968± 0.0909、0.4468 ± 0.1215.Ⅱ、Ⅲ组与Ⅰ组相比分别减少6.57%、16.16%.CA3区SDH的MOD值Ⅰ、Ⅱ、Ⅲ组分别为0.5668 ±0.1183、O.5042±0.1814、0,4182± 0.1196.Ⅱ、Ⅲ组与Ⅰ组相比分别减少
Alcohol is a kind of lipophilic small molecule substance which can rapidly act on the brain through the blood-brain barrier, and the hippocampus and cerebellum are the most sensitive neurotoxicity in the brain.In this study, Hydrogenase (SDH) was measured to investigate the effect of alcohol on brain energy metabolism.Methods 15 Kunming mice (20-30g) were divided into two groups: Ⅰ, Ⅱ and Ⅲ The rats in group Ⅲ were fed with pure water, 5% alcohol and 10% alcohol as sole drink for 60 days, respectively. Brain slices and vibrating microtome were sliced. The contents of SDH were determined by histochemical Peason method. The hippocampal CA_1 and CA_3 regions were detected by image analyzer The mean optical density (MOD) of SDH-positive reaction products showed that the MOD values of SDH in CA1 area were 0.5329 ± 0.0887,04968 ± 0.0909 and 0.4468 ± 0.1215, respectively Respectively, decreased by 6.57% and 16.16% respectively compared with that in group Ⅰ.The MOD values of group Ⅰ, Ⅱ and Ⅲ of SDH in CA3 area were 0.5668 ± 0.1183, 0.5042 ± 0.1814 and 0.4182 ± 0.1196, respectively Compared to reduce