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目的 制作先天性膈疝的动物模型 ,为进一步研究先天性膈疝合并肺发育不良的病理改变及其处理奠定基础。 方法 妊娠 9.5天的 SD大白鼠随机分成实验组和对照组。实验组经胃一次性灌入 2 ,4-二氯苯基 - P-硝基苯醚 ,对照组经胃灌入等量食用油。妊娠 2 1天时剖腹产取出胎鼠 ,放大镜下观察膈疝形成情况 ,并分别测定胎鼠体重及双肺重量 ,光学显微镜下观察肺的组织学发育情况。 结果 有 2 5只胎鼠制成先天性膈疝模型 ,成功率为 5 8.1% ,实验组胎鼠双肺重量降低 ,组织学显示肺发育不良。 结论 用 2 ,4-二氯苯基 - P-硝基苯醚制作胎鼠膈疝模型具有简便、成功率高的优点 ,是进一步研究膈疝合并肺发育不良的病理改变及其治疗的理想手段
Objective To make an animal model of congenital diaphragmatic hernia and lay the foundation for the further study of the pathological changes and treatment of congenital diaphragmatic hernia complicated with lung dysplasia. Methods SD rats of 9.5 days gestation were randomly divided into experimental group and control group. The experimental group was infused with 2,4-dichlorophenyl-p-nitrophenol through the stomach in one go, and the control group was infused with the same amount of cooking oil through the stomach. Twelve days of gestation, the fetus was removed by caesarean section and the formation of diaphragmatic hernia was observed under a magnifying glass. The weight of fetal rat and the weight of both lungs were measured respectively. The histopathological development of lung was observed under light microscope. Results Twenty-five congenital diaphragmatic hernia models were made, with a success rate of 5.181%. The weight of both lungs in the experimental group decreased and histology showed poor lung development. Conclusions It is an easy and successful method to make fetal rat diaphragmatic hernia model with 2,4-dichlorophenyl-p-nitrophenylether, which is an ideal method to further study the pathological changes of diaphragmatic hernia complicated with pulmonary dysplasia and its treatment