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目的:探讨苦参碱对膀胱癌大鼠环加氧酶2(cyclooxygenase-2,COX-2)、细胞膜磷脂酶A2(cytosolic phospholipases A2,c PLA2)、前列腺素脱氢酶(prostaglandin dehydrogenase,PGDH)蛋白表达水平的影响。方法:选取SD雄性大鼠120只,随机分为正常对照组、模型组、塞来昔布组、苦参碱高剂量组、苦参碱中剂量组、苦参碱低剂量组,各20只。给予亚硝基胺灌胃建立膀胱癌模型,并给予相应的药物治疗。观察所有大鼠的膀胱质量、质量>200 mg膀胱所占比例、肿瘤细胞凋亡指数,并对各组大鼠COX-2、c PLA2及PGDH蛋白的表达水平进行检测。结果:治疗后,与模型组比较,各药物组大鼠的膀胱质量、质量>200 mg膀胱所占比例较低,其中塞来昔布组、苦参碱高剂量组低于苦参碱中剂量组、低剂量组(P<0.05);治疗后,与模型组比较,各药物组大鼠肿瘤细胞凋亡指数较高,其中塞来昔布组、苦参碱高剂量组高于苦参碱中剂量组、苦参碱低剂量组(P<0.05);治疗后,与模型组比较,各药物组大鼠COX-2阳性表达率较低,其中塞来昔布组、苦参碱高剂量组低于苦参碱中剂量组、苦参碱低剂量组(P<0.05);治疗后,与模型组比较,各药物组大鼠COX-2、c PLA2蛋白表达水平较低,其中塞来昔布组、苦参碱高剂量组低于苦参碱中剂量组、苦参碱低剂量组(P<0.05);各药物组大鼠PGDH蛋白表达水平较高,其中塞来昔布组、苦参碱高剂量组高于苦参碱中剂量组、苦参碱低剂量组(P<0.05)。结论:苦参碱能够明显抑制亚硝基胺诱发的大鼠膀胱癌的生长,其作用机制可能与抑制前列腺素通路相关蛋白COX-2、c PLA2表达、促进PGDH蛋白表达有关,且其作用具有剂量依赖性。
Objective: To investigate the effects of matrine on cyclooxygenase-2 (COX-2), cytosolic phospholipase A2 (c PLA2), prostaglandin dehydrogenase (PGDH) Protein expression levels. Methods: 120 SD male rats were randomly divided into normal control group, model group, celecoxib group, matrine high dose group, matrine middle dose group, matrine low dose group, 20 . Given nitrosamines gavage to establish bladder cancer model, and give the appropriate drug treatment. All the rats were examined for bladder mass, mass> 200 mg of bladder, apoptosis index, and the expression of COX-2, c PLA2 and PGDH protein in each group were detected. Results: After treatment, compared with the model group, the urinary bladder mass and mass> 200 mg of bladder in each drug group were lower than those in the untreated group. The celecoxib group and the matrine high dose group were lower than the medium dose of matrine (P <0.05). After treatment, compared with the model group, the apoptotic index of tumor cells in each drug group was higher, in which the celecoxib group and matrine high dose group were higher than matrine (P <0.05). After treatment, compared with the model group, the positive rate of COX-2 expression in each drug group was lower, in which the celecoxib group, matrine high dose (P <0.05). After treatment, compared with the model group, the expression of COX-2 and c PLA2 protein in each drug group was lower, and the content of PLA2 in the drug group was lower than that in the matrine low dose group Compared with the matrine middle dose group and the matrine low dose group (P <0.05), the expression of PGDH protein in each drug group was significantly higher in the celecoxib group, the celecoxib group, the celecoxib group, the celecoxib group, The matrine high dose group was higher than the matrine middle dose group and matrine low dose group (P <0.05). CONCLUSION: Matrine can significantly inhibit the growth of rat bladder cancer induced by nitrosamine, and its mechanism may be related to the inhibition of the expression of prostaglandin pathway related protein COX-2, c PLA2 and the promotion of PGDH protein expression, and its action has Dose-dependent.