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为了从细胞水平研究低频电磁场影响成骨样细胞增殖的有效物理参数 ,并为其作用机制的解释提供依据 ,论文就几种类型电磁场对成骨样细胞 UMR- 10 6DNA合成的作用进行了一系列实验 ,通过 3 H-脱氧胸苷掺入检测 DNA合成的改变 ,发现特定频率、场强 (或磁感应强度 )组合的电磁场 (脉宽 0 .2 m s,10 V/cm,12 5 Hz附近的脉冲电场 ;1V/cm,10 Hz附近的交变电场 ;0 .5 m T,5 Hz附近的交变磁场 )能促进细胞 DNA合成水平显著提高 ,表明刺激骨细胞生长的电磁场无需复杂的波形。场强对 DNA合成有重要作用 ,只有在合适的场强范围内 ,一定频率的电磁场对细胞才有刺激作用。交变磁场所诱导的电场很微弱 ,表明磁场不只是通过诱导电场发挥作用的 ,研究磁场对细胞的作用应考虑包括磁场直接作用等全部可能的机制。
In order to study the effective physical parameters of osteoblast-like cell proliferation by low frequency electromagnetic field at the cellular level and provide a basis for the explanation of its mechanism of action, a series of studies on the effect of several types of electromagnetic fields on the synthesis of osteoblast-like UMR- Experiments were performed to detect changes in DNA synthesis by incorporation of 3 H-deoxythymidine into the presence of a combination of electromagnetic fields of specific frequency, field intensity (or magnetic induction intensity) (pulse width 0.2 ms, 10 V / cm, pulses at 125 Hz Electric field; 1V / cm, alternating electric field near 10 Hz; alternating magnetic field at 0. 5 mT, 5 Hz) promoted the DNA synthesis of cells significantly increased, indicating that electromagnetic fields stimulating the growth of osteocytes do not need complex waveforms. Field strength plays an important role in DNA synthesis, and only in a suitable range of field strength, a certain frequency of the electromagnetic field has a stimulating effect on the cells. The electric field induced by the alternating magnetic field is very weak, indicating that the magnetic field does not act only by inducing the electric field. It is necessary to consider all the possible mechanisms including the direct effect of the magnetic field on the action of the magnetic field on the cell.