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目的:建立HPLC-UV-ELSD联用法对双黄连系列制剂中连翘含量进行测定,并同时进行山银花的检查,在有效控制连翘质量的同时,考察双黄连系列制剂中金银花药材的投料情况。方法:采用相同色谱条件,不同检测器同时测定连翘苷含量与灰毡毛忍冬皂苷乙,HPLC-UV法测定连翘药材中连翘苷的含量,HPLC-ELSD测定灰毡毛忍冬皂苷乙,对金银花药材和山银花药材进行区别。结果:在2种双黄连系列固体制剂中检出山银花。结论:所建立的方法简便、准确,能够准确测定连翘苷的含量,并判断处方中金银花药材的投料应用情况。
OBJECTIVE: To establish a HPLC-UV-ELSD combined method for the determination of forsythia suspense in Shuanghuanglian series preparations and check the lesser. To effectively control the quality of Forsythia suspensa, Happening. Methods: The content of forsythin in Forsythia suspensa was determined by the same chromatographic conditions, the content of forsythin and the content of forsythia berberine were determined by different detectors at the same time, the content of forsythin in forsythia was determined by HPLC-UV, Differences between honeysuckle herbs and medicinal herbs. Results: Shan Yaohua was detected in two kinds of solid preparations of Shuanghuanglian series. Conclusion: The established method is simple and accurate, and can accurately determine the content of forsythin, and determine the application of honeysuckle herbs prescription.