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为探讨Tbx18+祖细胞在小鼠生长发育过程中的多分化潜能及分化的组织类型,本工作建立了Tbx18:Cre/Rosa26RLac Z谱系示踪小鼠.该示踪小鼠基于Cre-LoxP系统,能够准确及有效地示踪Tbx18+祖细胞的分化命运,通过整体胚胎及组织X-gal染色,检测分析报告基因LacZ在其中的表达情况.结果显示,在Tbx18:Cre/Rosa26RLac Z双杂合小鼠胚胎发育早期,报告基因LacZ主要在脊柱、四肢及心外膜表达;而在胚胎发育晚期则分别表达于皮肤、毛囊、肾脏、输尿管、膀胱、睾丸、输精管、椎间盘、肋软骨、心耳、心肌、冠状动脉.结果阐明,Tbx18+祖细胞在小鼠生长发育过程中具有强大的多器官及组织分化潜能,包括分化形成表皮系统,泌尿生殖系统,骨骼系统,心血管系统,并在其生长发育中发挥重要作用.
In order to investigate the multi-differentiation potential and differentiation of Tbx18 + progenitor cells in mouse growth and development, a Tbx18: Cre / Rosa26RLac Z-tracing mouse was established in this study.This mouse was based on the Cre-LoxP system Accurate and effective tracking of the fate of Tbx18 + progenitor cells, X-gal staining of whole embryos and tissues was used to detect the expression of reporter gene LacZ in Tbx18: Cre / Rosa26RLac Z double hybrid mouse embryos In early development, the reporter gene LacZ mainly expressed in the spine, extremities and epicardium, while in late embryos it was expressed in the skin, hair follicle, kidney, ureter, bladder, testis, vas deferens, disc, costal cartilage, atrial appendage, myocardium, Arteries The results show that Tbx18 + progenitor cells have a strong multi-organ and tissue differentiation potential during mouse growth and development, including differentiation into the epidermal system, genitourinary system, skeletal system, and cardiovascular system and play an important role in their growth and development effect.