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目的研究禾叶风毛菊醇提物(EESGD)对四氯化碳(CCl4)致肝损伤小鼠的保护作用。方法按照体重将小鼠随机分为6组:正常组、对照组(联苯双酯溶液150 mg·kg~(-1))、模型组和高、中、低3个剂量实验组(EESGD:4,2,1g·kg~(-1))。用腹腔注射0.15%CCl4花生油溶液复制肝损伤模型。造模16 h后,摘眼球取血。用速率法检测血清中的谷丙转氨酶(ALT)、谷草转氨酶(AST)的含量,用酶联免疫吸附法(ELISA)检测血清中肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)的含量。取血后剖取肝,用比色法检测肝组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽(GSH)的含量。结果给药后,正常组、对照组、模型组和高、中、低3个剂量实验组的ALT值分别为(14.90±3.35),(28.11±12.55),(47.38±20.15),(30.22±16.63),(32.67±11.67),(43.38±25.91)U·L~(-1);这6组的AST值分别为(97.75±11.96),(99.00±9.50),(129.86±17.67),(94.50±11.56),(96.86±13.06),(112.29±15.30)U·L~(-1);这6组的TNF-α值分别为(382.92±82.01),(626.79±142.09),(914.69±157.98),(425.89±149.40),(756.87±76.49),(654.79±131.54)pg·m L~(-1);这6组的IL-6值分别为(18.44±2.23),(24.14±9.01),(58.13±8.68),(34.20±11.65),(34.73±13.40),(42.15±19.56)pg·m L~(-1);这6组的SOD值分别为(91.62±3.92),(85.06±7.94),(48.86±2.3),(86.15±6.05),(66.04±7.83),(54.74±7.77)U·mg-1;这6组的MDA值分别为(0.31±0.04),(0.68±0.14),(0.83±0.08),(0.58±0.16),(0.77±0.08),(0.82±0.17)nmol·mg-1;这6组的GSH值分别为(10.36±0.38),(9.70±0.56),(8.00±0.43),(9.92±0.73),(8.74±0.72),(8.78±0.65)mg·g-1。与模型组相比,中、高2个剂量实验组可显著降低肝损伤小鼠血清中ALT、AST的含量,差异均有统计学意义(P<0.05,P<0.01);3个剂量实验组均可显著降低肝损伤小鼠血清中TNF-α和IL-6的含量,均可显著升高肝损伤小鼠的肝匀浆中SOD、GSH水平,差异均有统计学意义(P<0.05,P<0.01)。高剂量实验组可明显降低MDA水平,差异有统计学意义(P<0.01)。结论 EESGD对四氯化碳所致小鼠肝损伤有一定的保护作用。
Objective To study the protective effect of Eisenberg sativa extract (EESGD) on liver injury induced by carbon tetrachloride (CCl4) in mice. Methods According to body weight, mice were randomly divided into 6 groups: normal group, control group (bifendate solution 150 mg · kg -1), model group and high, middle and low doses of three experimental groups (EESGD: 4, 2, 1 g · kg -1). The model of hepatic injury was duplicated by intraperitoneal injection of 0.15% CCl4 peanut oil solution. After modeling for 16 h, the eyes were taken for blood. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were detected by the rate method. Serum levels of tumor necrosis factor alpha (TNF-α) and interleukin-6 IL-6) content. The liver was taken after the blood was taken and the content of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) in the liver homogenate were detected by colorimetry. Results The ALT of the normal, control, model and high, middle and low dose groups were (14.90 ± 3.35), (28.11 ± 12.55), (47.38 ± 20.15) and (30.22 ± 16.63), (32.67 ± 11.67) and (43.38 ± 25.91) U · L -1, respectively. The AST values of the six groups were (97.75 ± 11.96), (99.00 ± 9.50), (129.86 ± 17.67) and (96. 86 ± 13.06) and (112.29 ± 15.30) U · L ~ (-1) respectively. The TNF-α values of the 6 groups were (382.92 ± 82.01), (626.79 ± 142.09) and (914.69 ± The values of IL-6 in the six groups were (18.44 ± 2.23), (24.14 ± 9.01), (96.89 ± 149.40), (756.87 ± 76.49), (654.79 ± 131.54) pg · m L -1 ), (58.13 ± 8.68), (34.20 ± 11.65), (34.73 ± 13.40) and (42.15 ± 19.56) pg · m L -1 .The SOD values of these six groups were (91.62 ± 3.92), 85.06 ± 7.94), (48.86 ± 2.3), (86.15 ± 6.05), (66.04 ± 7.83) and (54.74 ± 7.77) U · mg-1 respectively.The MDA values in these 6 groups were (0.31 ± 0.04) and ± 0.14), (0.83 ± 0.08), (0.58 ± 0.16), (0.77 ± 0.08) and (0.82 ± 0.17) nmol · mg-1 respectively.The GSH values in these 6 groups were (10.36 ± 0.38) and 0.56), (8.00 ± 0.43), (9.92 ± 0.73), (8.74 ± 0.72) and (8.78 ± 0.65) mg · g-1, respectively. Compared with the model group, the middle and high doses of two doses of the experimental group can significantly reduce the serum levels of ALT and AST, the differences were statistically significant (P <0.05, P <0.01); three doses of the experimental group Could significantly reduce the content of TNF-α and IL-6 in the liver of mice with liver injury, both of which could significantly increase the levels of SOD and GSH in the liver homogenate of mice with liver injury, the difference was statistically significant (P <0.05, P <0.01). High-dose experimental group can significantly reduce MDA levels, the difference was statistically significant (P <0.01). Conclusion EESGD can protect CCl4-induced liver injury in mice.