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目的利用单侧横突拉簧加压法建立兔脊柱侧凸模型。方法两月龄新西兰白兔10只,手术予L2、L5右侧横突挂载拉簧。术前、术后1、4及8周摄X线正侧位片,测量Cobb角及拉簧长度,术后8周摄片后,随机将10只新西兰白兔分为两组:取拉簧(Spring removal,SR)组和保留拉簧组(Spring keeping,SK)各5只,取出SR组兔子体内拉簧,于9周和12周摄X线正侧位片,测量Cobb角及后凸角。12周后取所有兔顶椎及其上线椎体行Micro-CT扫描,记录顶椎上下终板的骨体积分数(BV/TV)。用方差分析或非参数检验及Student t检验对实验结果进行统计学分析。结果至实验结束时,兔全部存活,Cobb角均大于10°。术后1、4、8周不同时间点Cobb角及后凸角都大于术前(P<0.05)。术后8周,SR组与SK组的Cobb角、后凸角以及拉簧长度均无明显差异(P>0.05),取出拉簧后至12周,SK组的Cobb角及后凸角都较SR组要大(P<0.05),期间SR组的Cobb角及后凸角都逐渐减小。Micro-CT结果示凹侧终板骨体积分数要大于凸侧。整个实验过程中,拉簧形变量(ΔL)均无统计学差异(P>0.05)。结论单侧横突拉簧加压模型是一种可重复率高、简单易操作且实验周期短的动物脊柱侧凸模型。
Objective To establish a model of scoliosis in rabbits by compression with unilateral transverse process tension spring. Methods Ten New Zealand white rabbits of two months old were enrolled in the study. Preoperative, postoperative 1, 4 and 8 weeks after radiography X-ray lateral radiographs were measured Cobb angle and tension length, 8 weeks after radiography, 10 New Zealand white rabbits were randomly divided into two groups: take tension spring 5 rabbits in each group were divided into two groups: the spring removal (SR) group and the spring keeping (SK) group. The tension spring was removed from the rabbits in SR group. X-ray films were taken at 9 weeks and 12 weeks. Cobb angle and kyphosis angle. All rabbits and their upper vertebral bodies were scanned by Micro-CT after 12 weeks, and the volume fraction of bone (BV / TV) of the apical vertebrae was recorded. The results of variance analysis or nonparametric test and Student’s t test were used for statistical analysis. Results At the end of the experiment, all rabbits survived, with Cobb angles greater than 10 °. At 1, 4 and 8 weeks after operation, the Cobb angle and kyphotic angle were all higher than those before operation (P <0.05). At 8 weeks after operation, there was no significant difference in Cobb angle, kyphosis angle and extension length between SK group and SR group (P> 0.05). Cobb angle and kyphotic angle in SK group were significantly higher than those in SK group SR group was larger (P <0.05), Cobb angle and kyphotic angle of SR group decreased gradually during SR group. Micro-CT results showed that the concave side of the end plate bone volume fraction is greater than the convex side. Throughout the experiment, there was no significant difference (ΔL) between the tension spring deformations (ΔL) (P> 0.05). Conclusion The unilateral transverse process tension spring compression model is an animal model of scoliosis with high reproducibility, simple operation and short experimental period.