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目的 :观察喉癌组织及其引流淋巴结中巨噬细胞 (Mphi)表达与转移的关系。 方法 :采用免疫组化卵白素 -生物素过氧化酶复合物技术 (ABC法 ) ,对 6 0例喉癌患者的癌组织及其引流淋巴结石蜡切片中Mphi数目及形态进行检测。结果 :未发生颈淋巴结转移的喉癌组织及其引流淋巴结中Mphi数目明显多于转移者 (P <0 .0 1) ;且Mphi数目与临床分期有关 ,即随着病变范围的扩大 ,Mphi数目呈减少趋势。Mphi形态在未转移组中以多突形和梭形居多 ,在转移组中以小圆形细胞居多。结论 :喉癌患者癌组织及其引流淋巴结中Mphi的表达与喉癌的转移及发展有关 ,故Mphi检测对了解喉癌患者机体免疫状态、预测转移及评估喉癌预后有一定意义。
Objective: To observe the relationship between the expression of Mphi and metastasis in laryngeal carcinoma and its draining lymph nodes. Methods: Immunohistochemistry of avidin - biotin peroxidase complex (ABC method) was used to detect the number and morphology of Mphi in 60 cases of laryngeal carcinoma and their paraffin drainage paraffin sections. Results: The number of Mphi in laryngeal carcinoma without lymph node metastasis and its draining lymph nodes were significantly higher than those with metastasis (P <0.01). The number of Mphi was related to clinical stage, that is, with the extension of the lesion, the number of Mphi Showing a decreasing trend. Mphi morphology in the non-metastasis group to more protruding and spindle-shaped mostly in the transfer group to the majority of small round cells. Conclusion: Mphi expression in laryngeal cancer tissues and its draining lymph nodes is related to the metastasis and development of laryngeal cancer. Therefore, Mphi detection is of certain significance in understanding the immune status of laryngeal cancer patients, predicting the metastasis and assessing the prognosis of laryngeal cancer.