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AIM:To explore the effects of COX-2 gene in theproliferative activity induced by bile from anomalouspancreaticobiliary ductal union (APBDU) on humancholangiocarcinoma cell line.METHODS:Bile sample from APBDU and normal bilesample were used for this study.The proliferative effect ofbile was measured by methabenzthiazuron (MTT) assay;COX-2 mRNA was examined by semi-quantitative reversetranscription polymerase chain reaction (RT-PCR).Cell cyclewas analyzed by flow cytometry (FCM),and the PGE_2 levelsin the supernatant of cultured cholangiocarcinoma cellswere quantitated by enzyme-linked immunoabsordentassay (ELISA).RESULTS:Bile from APBDU can significantly promotethe proliferation of human cholangiocarcinoma QBC939cells compared with normal bile (P=0.005) and up-regulated remarkably their COX-2 mRNA expression(P=0.004).The proliferative activity of APBDU bile canbe abolished by addition of cyclooxygenase-2 specificinhibitor celecoxib.CONCLUSION:Bile from APBDU can promote theproliferation of human cholangiocarcinoma QBC939 cells viaCOX-2 pathway.
AIM: To explore the effects of COX-2 gene in the proliferative activity induced by bile from anomalous pancreaticobiliary ductal union (APBDU) on humancholangiocarcinoma cell line. METHODS: Bile sample from APBDU and normal bilesample were used for this study. The proliferative effect ofbile was measured by methabenzthiazuron (MTT) assay; COX-2 mRNA was examined by semi-quantitative reversetranscription polymerase chain reaction (RT-PCR). Cell cycle was analyzed by flow cytometry (FCM), and the PGE_2 levels in the supernatant of cultured cholangiocarcinoma cellswere quantitated by enzyme RESULTS: The proliferation activity of the human cholangiocarcinoma QBC939 cells compared with normal bile (P = 0.005) and up-regulated remarkably their COX-2 mRNA expression (P = 0.004) APBDU bile canbe abolished by addition of cyclooxygenase-2 specific inhibitor celecoxib.CONCLUSION: Bile from APBDU can promote theproliferation of human cholangiocarcinoma QBC939 cells viaCOX-2 pathway.