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目的探讨甘露聚糖结合凝集素(Mannan-binding lectin,MBL)对CD11c+髓样树突状细胞(CD11c+m DC)表型和功能的影响。方法应用磁珠分选技术获得BALB/c小鼠脾脏CD11c+m DC和CD4+T淋巴细胞。在CD11c+m DC中加入不同浓度的MBL(2.5~20μg/m L)刺激,以不加MBL的细胞作为对照,应用ELISA法检测细胞培养上清液中的IL-12水平,流式细胞仪检测细胞表面分子CD40、CD80、CD86及HLADR的表达。用MTT法测定CD11c+m DC刺激CD4+T淋巴细胞的增殖能力。ELISA法检测细胞培养液中IL-4和IFN-γ水平。结果 MBL显著增强CD11c+m DC表面分子CD40、CD80、CD86及HLA-DR的表达和IL-12的分泌,促进CD4+T淋巴细胞的增殖和抗原递呈能力,诱导CD4+T向TH1反应分化。结论 MBL能够有效刺激CD11c+m DC的活化,诱导CD4+T淋巴细胞向TH1反应分化。
Objective To investigate the effect of Mannan-binding lectin (MBL) on the phenotype and function of CD11c + myeloid dendritic cells (CD11c + m DC). Methods The spleen CD11c + m DC and CD4 + T lymphocytes of BALB / c mice were obtained by magnetic bead sorting. Different concentrations of MBL (2.5 ~ 20μg / m L) were added to CD11c + m DC to stimulate, and MBL-free cells were used as control. IL-12 level in cell culture supernatant was detected by ELISA. Flow cytometry The expression of cell surface molecules CD40, CD80, CD86 and HLADR were detected. The proliferation of CD4 + T lymphocytes stimulated by CD11c + m DC was measured by MTT assay. The levels of IL-4 and IFN-γ in cell culture medium were detected by ELISA. Results MBL significantly enhanced the expression of CD40, CD80, CD86 and HLA-DR on the surface of CD11c + m DCs and the secretion of IL-12, promoted the proliferation and antigen presentation of CD4 + T lymphocytes and induced the differentiation of CD4 + T to TH1 . Conclusion MBL can effectively stimulate the activation of CD11c + m DC and induce CD4 + T lymphocytes to differentiate into TH1.