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由于难以在体外用HBV感染人肝脏细胞且缺乏理想的动物模型,因此对HBV引起的肝细胞坏死及机体清除HBV的机制仍不很清楚。作者建立了一个体内转染人HBV感染的动物模型,现报道如下。 借助能促进细胞膜融合的阳离子脂,双十八烷基酰胺甘氨酰精胺,直接将克隆有HBV adw亚型整个基因组序列的质粒载体导入7～8周龄的S-D CD大鼠的肝脏。为了增加转移基因的表达,在转染前24小时对大鼠行部分(2/3)肝切除术。
Because it is difficult to in vitro HBV infection of human liver cells and the lack of ideal animal models, so the mechanism of liver cell necrosis caused by HBV and the body to clear HBV remains unclear. The authors have established an animal model of human HBV infection in vivo and are reported below. The plasmid vector containing the entire genomic sequence of the HBV adw subtype was directly introduced into the liver of S-D CD rats aged 7-8 weeks by means of cationic lipid, dioctadecylamide glycyl spermine, which promotes cell membrane fusion. In order to increase the expression of the transgene, a partial (2/3) hepatectomy was performed on rats 24 hours prior to transfection.