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We cultured rat muscle-derived stem cells in medium containing nerve growth factor and basic fibroblast growth factor to induce neuronal-like cell differentiation. Immunocytochemical staining and reverse transcription-PCR showed that the differentiated muscle-derived stem cells exhibited processes similar to those of neuronal-like cells and neuron-specific enolase expression, but Notch1 mRNA and protein expression was decreased. Down-regulation of Notch1 expression may facilitate neuronal-like cell differentiation from muscle-derived stem cells.rn