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目的:研究灰褐纹口蘑发酵液的化学成分。方法:采用硅胶柱层析、反相RP-18柱层析、Sephadex LH-20柱等色谱技术进行分离和纯化,通过质谱、核磁共振波谱等方法对化合物结构进行鉴定。结果:从灰褐纹口蘑发酵液中分离鉴定了12个化合物,分别为:2,6,10-trimethyldodeca-6,11-dien-1,10-diol(1)、6-hydroxy-2,6-dimethyl-7-octenoic acid(2)、(3S)-3,7,11-trimethyldodeca-6,10-dien-1,2,3-triol(3)、4,8-dimethyl-docadiene-4,9-dien-1,8-diol(4)、3,10-epoxy-3,7,11-trimethyldodeca-1,6-dien-11-ol(5)、Cyclo(D)-Pro-(D)-Leu(6)、(22E,24R)-5α,8α-epidioxy-ergosta-6,22-dien-3β-ol(7)、(22E,24R)-ergosta-5,7,22-trien-3β-ol(8)、(22E,24R)-ergosta-7,22-dien-3β,5α,6β-triol(9)、(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(10)、5-hydroxymethyl-2-furancarboxaldehyde(11)、4-hydroxyphenethyl alcohol(12)。结论:其中,化合物1为新化合物,化合物2~5为从该属真菌中首次分离得到,所有化合物均为从该菌中首次分离得到。
Objective: To study the chemical constituents of the fermentation broth of Tricholoma matsutake. Methods: The compounds were isolated by silica gel column chromatography, reverse phase RP-18 column chromatography and Sephadex LH-20 column chromatography. The structures of the compounds were identified by mass spectrometry and nuclear magnetic resonance spectroscopy. Results: Twelve compounds were isolated and identified from the fermentation broth of Mucor rubidium, which were 2,6,10-trimethyldodeca-6,11-dien-1,10-diol (1), 6-hydroxy-2,6 -dimethyl-7-octenoic acid (2), (3S) -3,7,11-trimethyldodeca-6,10-dien- 1,2,3-triol (3) 9-dien-1,8-diol (4), 3,10-epoxy-3,7,11-trimethyldodeca-1,6-dien- -Leu (6), (22E, 24R) -5α, 8α-epidioxy-ergosta-6,22-dien-3β- ol (7), (22E, 24R) -ergosta-5,7,22-trien-3β (22E, 24R) -ergosta-7,22-dien-3β, 5α, 6β- triol (9), (22E, 24R) -ergosta-4,6,8 (14), 22- tetraen-3-one (10), 5-hydroxymethyl-2-furancarboxaldehyde (11), 4-hydroxyphenethyl alcohol (12). Conclusion: Among them, compound 1 is a new compound, and compounds 2 ~ 5 are isolated from the fungi for the first time. All the compounds were isolated from this strain for the first time.