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目的 观察不同强度恒磁场对氧化修饰低密度脂蛋白 (OX LDL)作用下人脐静脉内皮细胞 (HUVEC)分泌和表达细胞间黏附分子 1(ICAM 1)及HUVEC与人单核细胞株THP 1黏附的影响。方法 采用体外培养第 3代的HUVEC ,实验分为 6组 ,即对照组、OX LDL(10 0mg·L-1)组及OX LDL +不同磁感应强度 (1、5、10、2 0Gs)的恒磁场组。各组细胞于培养及恒磁场作用 2 4h后收集标本 ,用酶联免疫吸附试验 (ELISA)检测HUVEC分泌ICAM 1的量 ,免疫细胞化学检测ICAM 1的表达 ,计数法观察HUVEC与THP 1的黏附率。结果 OX LDL 10 0mg·L-1刺激细胞 2 4h,ICAM 1的分泌和表达显著增高(P <0 .0 5vs.对照组 ) ;而 1、5、10和 2 0Gs恒磁场组细胞ICAM 1的分泌和表达显著低于OX LDL组 (P <0 .0 5 )。OX LDL10 0mg·L-1与HUVEC孵育 2 4h后 ,HUVEC与THP 1的黏附率显著增加 (P <0 .0 5vs.对照组 ) ;而 1、5、10和 2 0Gs恒磁场组HUVEC与THP 1的黏附率显著低于OX LDL组 (P <0 .0 5 )。结论 1～ 2 0Gs的恒磁场可拮抗OX LDL的作用 ,抑制HUVEC分泌和表达ICAM 1及与单核细胞的黏附率
Objective To observe the effects of different intensity constant magnetic fields on the secretion and expression of intercellular adhesion molecule 1 (ICAM 1) and HUVEC and human monocytic cell line THP 1 under the action of oxidized low density lipoprotein (OX LDL) in human umbilical vein endothelial cells Impact. Methods The third generation of HUVECs were cultured in vitro. The experiment was divided into 6 groups: control group, OX LDL group (10 0 mg · L -1) and OX LDL + group with different magnetic flux density (1,5,10,2 0Gs) Magnetic field group. The cells were harvested at 24 h after culture and constant magnetic field. The amount of ICAM-1 secreted by HUVECs was detected by enzyme-linked immunosorbent assay (ELISA), the expression of ICAM-1 by immunocytochemistry and the adhesion of HUVEC to THP1 rate. Results OX LDL 10 0 mg · L-1 stimulated cells for 24 h, the secretion and expression of ICAM 1 were significantly increased (P <0.05 vs control); while those in 1, 5, 10 and 20 Gs groups Secretion and expression were significantly lower than those in the OX LDL group (P <0.05). OX LDL10 0mg · L-1 and HUVEC incubated for 2 4h, HUVEC and THP 1 adhesion rate was significantly increased (P <0 05vs control group); and 1, 5, 10 and 20Gs constant magnetic field group HUVEC and THP 1 adhesion rate was significantly lower than the OX LDL group (P <0. 05). Conclusion 1 ~ 20Gs constant magnetic field can antagonize the role of OX LDL, inhibit the secretion of HUVEC and express ICAM 1 and monocyte adhesion rate