氮离子束注入和钴60伽玛辐射对大豆产量性状和品质的影响

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分别以3种剂量的N离子束注入(4×1016,6×1016和8×1016N+·cm-2)和3种剂量的60Co-γ射线辐射(100,150和200 Gy)处理4份大豆干种子,在相同种植条件下对其M6代产量性状和品质进行研究,比较2种处理方法对大豆产量性状及品质的诱变效应。结果表明:与对照相比,新大豆3号4×1016N+·cm-2离子束注入处理单株荚数显著增加,6×1016N+·cm-2离子束注入处理产量极显著增加;合99-756 8×1016N+·cm-2离子束注入处理单株粒数和单株粒重显著减少,6×1016N+·cm-2离子束注入处理百粒重显著减少,100 Gy60Co-γ射线辐射处理蛋白质含量显著降低,100,150和200 Gy60Co-γ射线辐射使脂肪含量显著提高;新大豆8号4×1016和8×1016N+·cm-2离子束注入、100和200 Gy60Co-γ射线辐射处理单株粒重显著减少,4×1016和6×1016N+·cm-2离子束注入及200 Gy60Co-γ射线辐射处理产量极显著或显著增加;N离子束注入和60Co-γ辐射对石大豆2号产量、各产量构成因素及品质的影响差异不显著。因此,诱变育种目标应根据材料的敏感性、诱变方法和剂量综合确定。 Four dried soybean seeds were treated with 3 doses of N ion beam implantation (4 × 1016, 6 × 1016 and 8 × 1016 N + · cm-2) and 3 doses of 60Co-γ ray irradiation (100, 150 and 200 Gy) The yield and quality of M6 generation under the same planting conditions were studied. The mutagenic effects of two treatments on yield and quality of soybean were compared. The results showed that compared with the control, the pod number of the new soybean No. 3 with 4 × 1016N + · cm-2 ion beam injection significantly increased, and the yield of 6 × 1016N + · cm-2 ion beam injection significantly increased. Compared with the control, the yield of 99-756 The grain weight per plant and grain weight per plant were significantly decreased by 8 × 1016N + · cm-2 ion beam implantation, and the 100-grain weight was significantly decreased by 6 × 1016N + · cm-2 ion beam implantation. The protein content of 100 Gy60Co-γ-ray irradiation treatment was significantly At 100, 200, and 60 Gy γ-rays, the grain weight of 60Co-γ-rays was significantly decreased. The implantation of 4 × 1016 and 8 × 1016N + · cm- , 4 × 1016 and 6 × 1016N + · cm-2 ion implantation and 200 Gy-60Co-γ-ray irradiation significantly or significantly increased the yield of stone soybean No.2, the yield components And the quality of the difference is not significant. Therefore, mutagenic breeding objectives should be based on the sensitivity of the material, mutagenesis methods and dose synthesis to determine.
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