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目的检测子宫内膜癌组织中p-ERK-1、p-p38和ERK-1 mRNA表达情况并探讨其意义。方法采用原位分子杂交方法和免疫组织化学S-P法检测60例子宫内膜癌、32例正常子宫内膜组织中三者的表达情况。结果子宫内膜癌组织中p-ERK-1和p-p38表达定位于细胞核,ERK-1 mRNA的表达于细胞浆,其阳性表达率分别为68.33%(41/60)、55.00%(33/60)和78.33%(47/60),明显高于正常子宫内膜组织(P<0.05)。p-ERK-1、p-p38蛋白和ERK-1 mRNA的表达与淋巴结转移、临床分期及病理分期有关(P<0.05);在子宫内膜癌组织中p-ERK-1蛋白和ERK-1 mRNA表达存在一致性(P<0.05),三者之间表达呈正相关(P<0.05)。结论 p-ERK-1、p-p38蛋白和ERK-1 mRNA在子宫内膜癌呈高表达,且与子宫内膜癌的发生、发展和转移密切相关。
Objective To detect the expression of p-ERK-1, p-p38 and ERK-1 mRNA in endometrial carcinoma and to explore its significance. Methods In situ hybridization and immunohistochemical S-P method were used to detect the expression of three of 60 endometrial carcinomas and 32 normal endometrial tissues. Results The expression of p-ERK-1 and p-p38 in the endometrial carcinoma was localized in the nucleus and the expression of ERK-1 mRNA in the cytoplasm was 68.33% (41/60) and 55.00% (33 / 60) and 78.33% (47/60), respectively, which were significantly higher than those in normal endometrium (P <0.05). The expression of p-ERK-1, p-p38 protein and ERK-1 mRNA were correlated with lymph node metastasis, clinical stage and pathological stage (P <0.05). In endometrial carcinoma tissues, the expression of p-ERK- mRNA expression was consistent (P <0.05), the expression of the three was positively correlated (P <0.05). Conclusion The expressions of p-ERK-1, p-p38 protein and ERK-1 mRNA are highly expressed in endometrial carcinoma and closely related to the occurrence, development and metastasis of endometrial carcinoma.