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目的 :以体外原代培养的小脑颗粒细胞为对象 ,测定电磁脉冲辐照神经细胞前后细胞内LDH和培养上清中LDH ,CHE ,K+ 和Na+ 浓度及与时间的关系 ,研究电磁脉冲 (场强为 6× 10 4 V m)辐照后早期对小脑颗粒细胞损伤的可能机制。方法 :在培养 12～ 14d时 ,高场强EMP模拟源 (有界波模拟源 ) ,场强为 6× 10 4 V m ,脉冲上升时间为2 0ns,脉宽为 30 μs ,频率包含 0～ 10 0MHz ,以 2 .5次 min ,辐照 2min。并于辐照后 0 (即刻 ) ,1,6 ,12和 2 4h应用生化检测试剂盒测定细胞内和培养上清中LDH及培养上清中CHE ,K+ 和Na+ 浓度。结果 :电磁脉冲辐照后即刻就可引起培养上清LDH明显升高 ;辐照后 1h细胞内LDH明显降低 ,而培养上清中LDH ,CHE和K+ 明显升高 ;辐照后 6h细胞内LDH明显降低 ,而培养上清中LDH ,CHE ,K+ 和Na+ 明显升高 ;辐照后 12h细胞内LDH明显降低 ,培养上清中CHE ,K+ 和Na+ 明显升高 ;辐照后 2 4h所有上述指标基本恢复。结论 :电磁脉冲辐照后可损伤大鼠海马神经元细胞膜。
OBJECTIVE: To determine the concentrations of LDH, CHE, K + and Na + in LDH and culture supernatants before and after irradiation of electromagnetic pulses on cultured cerebellar granule cells in vitro and their relationship with time. The effects of electromagnetic pulse (field intensity 6 × 10 4 V m) on the cerebellar granule cells after irradiation. Methods: During 12 ~ 14 days of cultivation, high-field EMP simulation source (bounded-wave simulation source) with field intensity of 6 × 10 4 V m, pulse rise time of 20 ns, pulse width of 30 μs and frequency of 0 ~ 10 0MHz, with 2.5 times min, irradiation 2min. The concentrations of CHE, K + and Na + in LDH and culture supernatant of cells and culture supernatants were determined by biochemical detection kit at 0 (immediately), 1, 6, 12 and 24 hours after irradiation. Results: Immediately after irradiation with electromagnetic pulse, the LDH of the culture supernatant was significantly increased. LDH in the supernatant of the culture supernatant was significantly decreased at 1h after irradiation, while the levels of LDH, CHE and K + in the culture supernatant were significantly increased. The intracellular LDH While the levels of LDH, CHE, K + and Na + in the supernatant of the culture increased significantly; the LDH of the cells in the supernatant of the culture supernatant decreased significantly at 12h after irradiation and the levels of CHE, K + and Na + in the culture supernatant were significantly increased; Basic recovery. Conclusion: Irradiation of electromagnetic pulse can damage rat hippocampal neuron cell membrane.