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目的:研究白藜芦醇对肝脏损伤的保护作用。方法:分别建立硫代乙酰胺(TAA,50mg/kg,ip)和乙醇(45%～10ml/kg,ig)诱导小鼠肝脏损伤的模型,以血清丙氨酸氨基转移酶(ALT)活性和一氧化氮含量(NO)、肝匀浆丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性为指标,观察白藜芦醇对实验性肝脏损伤的保护作用。结果:白藜芦醇(15,30mg/kg,ig×7d)能明显降低分别用硫代乙酰胺和乙醇处理的小鼠血清ALT,并提高肝脏SOD活性。白藜芦醇(30mg/kg,ig×7d)还可降低血清NO和肝匀浆MDA含量。结论:白藜芦醇具有明显的保护肝作用,可通过增强抗氧化能力及抑制NO生成而拮抗硫代乙酰胺和乙醇的肝毒性。
Objective: To study the protective effect of resveratrol on liver injury. METHODS: A model of liver injury induced by thioacetamide (TAA, 50 mg/kg, ip) and ethanol (45% to 10 ml/kg, ig) was established, and serum alanine aminotransferase (ALT) activity was measured. Nitric oxide (NO) content, malondialdehyde (MDA) content in liver homogenate and superoxide dismutase (SOD) activity were used as indices to observe the protective effect of resveratrol on experimental hepatic injury. RESULTS: Resveratrol (15,30 mg/kg, ig×7d) significantly reduced the serum ALT in mice treated with thioacetamide and ethanol, and increased SOD activity in the liver. Resveratrol (30mg/kg, ig×7d) also reduced serum NO and liver homogenate MDA content. CONCLUSION: Resveratrol has obvious protective hepatic effects and can antagonize hepatotoxicity of thioacetamide and ethanol by enhancing antioxidative ability and inhibiting NO production.