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目的:研究蝙蝠葛多酚类生物碱(phenolic alkaloids of Menispermum dauricum,PAMD)对豚鼠乳头肌和兔窦房结优势起搏细胞动作电位的影响。方法:运用玻璃微电极记录豚鼠心室乳头肌动作电位,研究不同浓度PMAD(1,3,10μmol·L-1)对心室乳头肌动作电位时程(action potential duration,APD),有效不应期(effective refractory period,ERP),最大除极化速度(maximum upstroke velocity,Vmax),动作电位幅度(amplitude,APA),超射(overshoot,OS)的影响;运用玻璃微电极记录兔窦房结优势起搏细胞动作电位,研究不同浓度PAMD(1,3,10μmol·L-1)对窦房结细胞APD、最大舒张期电位(maximal diastolic potential,MDP),APA,Vmax,自发放电频率(rate of pacemaker firing,RPF)和舒张期去极化速度[velocity of diastolic(phase 4)depolarization,VDD]的影响。结果:在豚鼠右心室心肌乳头肌细胞中研究发现,PAMD可以剂量依赖性的显著延长APD和ERP,延缓Vmax。当浓度为10μmol·L-1时,PAMD对窦房结自律性具有轻度影响,这种作用与对照组比有显著差异。结论:PAMD对心室肌的作用强于其对窦房结的作用,这表明其具有成为理想的抗室性心律失常的药物的潜力。
Objective: To study the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on the action potentials of palliative cells in guinea pig papillary muscle and sinus node. Methods: The action potential of guinea pig ventricular papillary muscle was recorded by glass microelectrode. The effects of different concentrations of PMAD (1,3,10 μmol·L -1) on the action potential duration (APD), effective refractory period (ERP), maximum upstroke velocity (Vmax), action potential amplitude (APA) and overshoot (OS). The effect of using glass microelectrode to record the sinus node To investigate the effects of different concentrations of PAMD (1,3,10 μmol·L-1) on APD, maximal diastolic potential (MDP), APA, Vmax, rate of pacemaker firing, RPF, and velocity of diastolic (phase 4) depolarization, VDD]. Results: In guinea pig ventricular myocytes, we found that PAMD can prolong the APD and ERP dose-dependently and delay Vmax. When the concentration of 10μmol·L-1, PAMD had a slight effect on the sinus node self-regulation, this effect was significantly different from the control group. Conclusions: The effect of PAMD on ventricular muscles is stronger than their effect on sinoatrial node, suggesting its potential as an ideal anti-ventricular arrhythmia drug.